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Your Position: Home > Immune Checkpoint Proteins > BTLA > Human BTLA Protein

Human BTLA Protein

  • Synonym
    BTLA,CD272
  • Source
    Human BTLA, His Tag (BTA-H52E0) is expressed from human 293 cells (HEK293). It contains AA Lys 31 - Ser 150 (Accession # AAP44003).
    Predicted N-terminus: Lys 31
  • Molecular Characterization
    BTLA(Lys 31 - Ser 150) AAP44003

    This protein carries a polyhistidine tag at the C-terminus, and has a calculated MW of 15.6 kDa. The predicted N-terminus is Lys 31. The reducing (R) protein migrates as 27-40 kDa in SDS-PAGE due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human BTLA, His Tag (Cat. No. BTA-H52E0) SDS-PAGE gel

Human BTLA, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Human BTLA, His TagHuman BTLA, His Tag (Cat. No. BTA-H52E0) ELISA bioactivity

Immobilized Human BTLA, His Tag (Cat.No. BTA-H52E0) at 5 μg/mL (100 μL/well) can bind Human HVEM, Fc Tag (Cat.No. HVM-H5258) with a linear range of 0.08-10 μg/mL (QC tested).

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