>IL23A & IL12B
>Human IL-23 alpha & IL-12 beta Heterodimer Protein
Human IL-23 alpha & IL-12 beta Heterodimer Protein, produced by co-expression of IL-23 alpha and IL-12 beta, has a calculated MW of 19.5 kDa (IL-23 alpha) and 34.7 kDa (IL-12 beta). Subunit IL-23 alpha is fused with a polyhistidine tag at the N-terminus and subunit IL-12 beta contains no tag. The predicted N-terminus is His (IL23A) & Ile 23 (IL12B). The reducing (R) protein migrates as 20 kDa (IL-23 alpha) and 37-45 kDa (IL-12 beta) respectively due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
Human IL23A & IL12B, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Captured Human IL-23 R, Fc Tag (Cat. No. ILR-H5254) on CM5 chip via anti-human IgG Fc antibodies surface can bind Human IL23A & IL12B, His Tag (Cat. No. ILB-H52W5) with an affinity constant of 5.36 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Human IL-23 alpha & IL-12 beta Heterodimer Protein(Cat. No. ILB-H52W5) stimulates secretion of IL-17 by mouse spleen cells. The ED50 for this effect is 0.1055-0.1635 ng/ml (Routinely tested).
Cell based assay shows that the secretion of IL-17 induced by Human IL-23 alpha & IL-12 beta Heterodimer Protein (Cat. No. ILB-H52W5) is inhibited by increasing concentration of the anti-human IL-23 neutralizing antibody. The IC50 is between 0.67-1.50 ng/mL (Routinely tested).
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Price(USD) : $350.00
Price(USD) : $3500.00
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