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Your Position: Home > Cytokines > IL23A & IL12B > Human IL-23 alpha & IL-12 beta Heterodimer Protein

Human IL-23 alpha & IL-12 beta Heterodimer Protein

  • Synonym
    IL-23 alpha & IL-12 beta
  • Source
    Human IL23A & IL12B, His Tag (ILB-H52W5) is expressed from human 293 cells (HEK293). It contains AA Arg 20 - Pro 189 (IL23A) & Ile 23 - Ser 328 (IL12B) (Accession # NP_057668 (IL23A) & AAH67499 (IL12B)).
    Predicted N-terminus: His (IL23A) & Ile 23 (IL12B)
  • Molecular Characterization
    Online(Arg 20 - Pro 189 (IL23A) & Ile 23 - Ser 328 (IL12B)) NP_057668 (IL23A) & AAH67499 (IL12B)

    Human IL-23 alpha & IL-12 beta Heterodimer Protein, produced by co-expression of IL-23 alpha and IL-12 beta, has a calculated MW of 19.5 kDa (IL-23 alpha) and 34.7 kDa (IL-12 beta). Subunit IL-23 alpha is fused with a polyhistidine tag at the N-terminus and subunit IL-12 beta contains no tag. The predicted N-terminus is His (IL23A) & Ile 23 (IL12B). The reducing (R) protein migrates as 20 kDa (IL-23 alpha) and 37-45 kDa (IL-12 beta) respectively due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Human IL23A & IL12B, His Tag (Cat. No. ILB-H52W5) SDS-PAGE gel

Human IL23A & IL12B, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Human IL23A & IL12B, His TagHuman IL23A & IL12B, His Tag (Cat. No. ILB-H52W5) ELISA bioactivity

Immobilized Human IL23A & IL12B, His Tag (Cat. No. ILB-H52W5) at 2 μg/mL (100 μL/well) can bind Anti IL23A&IL12B P40 domain, Human IgG1 with a linear range of 0.05-3 ng/mL (QC tested).


Captured Human IL-23 R, Fc Tag (Cat. No. ILR-H5254) on CM5 chip via anti-human IgG Fc antibodies surface can bind Human IL23A & IL12B, His Tag (Cat. No. ILB-H52W5) with an affinity constant of 5.36 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-Cell based assay
Human FcRn Heterodimer Protein Cell_Base

Human IL-23 alpha & IL-12 beta Heterodimer Protein(Cat. No. ILB-H52W5) stimulates secretion of IL-17 by mouse spleen cells. The ED50 for this effect is 0.1055-0.1635 ng/ml (Routinely tested).

Human FcRn Heterodimer Protein Cell_Base

Cell based assay shows that the secretion of IL-17 induced by Human IL-23 alpha & IL-12 beta Heterodimer Protein (Cat. No. ILB-H52W5) is inhibited by increasing concentration of the anti-human IL-23 neutralizing antibody. The IC50 is between 0.67-1.50 ng/mL (Routinely tested).

  • Background
    Interleukin-23 subunit alpha (IL-23 alpha) can associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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