The heterodimer Human CD3 epsilon & CD3 gamma is produced by co-expression of CD3E and CD3G, has a calculated MW of 39.2 kDa (CD3E) and 38.6 kDa (CD3G). Subunit CD3E is fused with a human IgG1 Fc tag at the C-terminus, followed by a polyhistidine tag, and subunit CD3G is fused with a human IgG1 Fc tag at the C-terminus, followed by a Flag tag. The predicted N-terminus is Asp 23 (CD3E) & Gln 23 (CD3G). The reducing (R) heterodimer protein migrates as 40-50 kDa due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
Human CD3E & CD3G, His Tag, Fc Tag, Flag Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized OKT3 MAb at 10μg/mL (100 μL/well) can bind Human CD3E & CD3G (Cat. No. CDG-H52W9) with a linear range of 0.3-5 μg/mL (Routinely tested).
Anti-Human CD3 mAb (OKT3), Ultra-low endotoxin (Cat. No. CDE-M120a) captured on CM5 chip via anti-mouse antibodies surface, can bind Human CD3E & CD3G, His Tag, Fc Tag, Flag Tag (Cat. No. CDG-H52W9) with an affinity constant of 3.16 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
SP34-2 (Mouse IgG1) captured on CM5 chip via anti-mouse antibodies surface, can bind Human CD3E & CD3G, His Tag, Fc Tag, Flag Tag (Cat. No. CDG-H52W9) with an affinity constant of 0.867 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
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