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Your Position: Home > Bio-Markers & CD Antigens > Human CD3 epsilon & CD3 gamma Protein

Human CD3 epsilon & CD3 gamma Heterodimer Protein

  • Synonym
    CD3 epsilon & CD3 gamma,CD3E & CD3G
  • Source
    Human CD3 epsilon & CD3 gamma (CDG-H52W9) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G) (Accession # NP_000724.1 (CD3E) & AAI13831 (CD3G)).
    Predicted N-terminus: Asp 23 (CD3E) & Gln 23 (CD3G)
  • Molecular Characterization
    CD3E & CD3G(Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G)) NP_000724.1 (CD3E) & AAI13831 (CD3G)

    The heterodimer Human CD3 epsilon & CD3 gamma is produced by co-expression of CD3E and CD3G, has a calculated MW of 39.2 kDa (CD3E) and 38.6 kDa (CD3G). Subunit CD3E is fused with a human IgG1 Fc tag at the C-terminus, followed by a polyhistidine tag, and subunit CD3G is fused with a human IgG1 Fc tag at the C-terminus, followed by a Flag tag. The predicted N-terminus is Asp 23 (CD3E) & Gln 23 (CD3G). The reducing (R) heterodimer protein migrates as 40-50 kDa due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human CD3 epsilon & CD3 gamma (Cat. No. CDG-H52W9) SDS-PAGE gel

Human CD3 epsilon & CD3 gamma on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Human CD3 epsilon & CD3 gammaHuman CD3 epsilon & CD3 gamma (Cat. No. CDG-H52W9) ELISA bioactivity

Immobilized OKT3 MAb at 10μg/mL (100 μL/well) can bind Human CD3E & CD3G (Cat. No. CDG-H52W9) with a linear range of 0.3-5 μg/mL (Routinely tested).

Human CD3 epsilon & CD3 gammaHuman CD3 epsilon & CD3 gamma (Cat. No. CDG-H52W9) ELISA bioactivity

Immobilized Mouse Anti Human CD3 at 5 μg/mL (100 μL/well) can bind Human CD3E & CD3G, His Tag, Fc Tag, Flag Tag (Cat. No. CDG-H52W9) with a linear range of 0.19-0.78 ng/mL (QC tested).

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Price(USD) : $2800.00

Inventory : In stock

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