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Your Position: Home > Protein > B7-H3 (2Ig) > B73-M5255

Mouse B7-H3 (2Ig) / CD276 Protein, Fc Tag (MALS verified)

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  • Source
    Mouse B7-H3 (2Ig) Protein, Fc Tag(B73-M5255) is expressed from human 293 cells (HEK293). It contains AA Val 29 - Phe 244 (Accession # NP_598744 ).
    Predicted N-terminus: Val 29
  • Molecular Characterization
    B7-H3 (2Ig) Structure

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 50.1 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
B7-H3 (2Ig) SDS-PAGE

Mouse B7-H3 (2Ig) Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
B7-H3 (2Ig) MALS images

The purity of Mouse B7-H3 (2Ig) Protein, Fc Tag (Cat. No. B73-M5255) is more than 90% and the molecular weight of this protein is around 105-125 kDa verified by SEC-MALS.

Bioactivity-ELISA
 B7-H3 (2Ig) ELISA

Immobilized Mouse B7-H3 (2Ig) Protein, Fc Tag (Cat. No. B73-M5255) at 1 μg/mL (100 μL/well) can bind Anti-B7-H3 Antibody, Mouse IgG1 with a linear range of 4-63 ng/mL (QC tested).

  • Background
    B7 homolog 3 (B7-H3), a member of the immunoglobulin superfamily, is also known CD276, which contains two Ig-like C2-type (immunoglobulin-like) domains and two Ig-like V-type (immunoglobulin-like) domains. B7-H3 may participate in the regulation of T-cell-mediated immune response. B7-H3 also plays a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. Furthermore, B7-H3 is involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. It could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy.
  • Clinical and Translational Updates

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