Human Galectin-9 / LGALS9 Protein

INTRODUCTION

QC & Assay Protocol

Citation & Background

Synonym
Galectin-9,LGALS9,Ecalectin
Source
Human Galectin-9, His Tag (LG9-H5244) is expressed from human 293 cells (HEK293). It contains AA Ala 2 - Thr 323 (Accession # BAA31542).
Predicted N-terminus: His
Request for sequence
Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 36.6 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE).
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 20 mM MOPS, 50 mM NaCl, 1 mM EDTA, 2 mM DTT, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
1. 4-8^°C for 12 months in lyophilized state;
2. -70^°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE

Human Galectin-9, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Background
Galectin-9 (LGALS9) is also known as tumor antigen HOM-HD-21 and ecalectin. Galectins are a family of proteins defined by their binding specificity for β-galactoside sugars, such as N-acetyllactosamine (Galβ1-3GlcNAc or Galβ1-4GlcNAc), which can be bound to proteins by either N-linked or O-linked glycosylation. As for Galectin-9, it has high affinity for the Forssman pentasaccharide, and it is also the ligand for HAVCR2/TIM3. Forthermore, Galectin-9 stimulates bactericidal activity in infected macrophages by causing macrophage activation and IL1B secretion which restricts intracellular bacterial growth.
References
- (1) Tuereci O., et al., 1997, J. Biol. Chem. 272:6416-6422.
- (2) Dai S.Y., et al.,2005, J. Immunol. 175:2974-2981.
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