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Your Position: Home > Immune Checkpoint Proteins > PD-L1 > PD1-M52A2

Mouse PD-L1 / B7-H1 Protein, mouse IgG2a Fc Tag, low endotoxin

INTRODUCTION
QC & Assay Protocol
Citation & Background
  • Synonym
    PD-L1,CD274,B7-H1,PDCD1L1,PDCD1LG1
  • Source
    Mouse PD-L1, Mouse IgG2a Fc Tag, low endotoxin (PD1-M52A2) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Thr 238 (Accession # NP_068693).
    Predicted N-terminus: Phe 19
    Request for sequence
  • Molecular Characterization
    Online(Phe 19 - Thr 238) NP_068693

    This protein carries a mouse IgG2a Fc tag at the C-terminus.

    The protein has a calculated MW of 51.8 kDa. The protein migrates as 65-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Mouse PD-L1, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. PD1-M52A2) SDS-PAGE gel

Mouse PD-L1, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Mouse PD-L1, Mouse IgG2a Fc Tag, low endotoxinMouse PD-L1, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. PD1-M52A2) ELISA bioactivity

Immobilized Mouse B7-1, Fc Tag (Cat. No. CD0-M5259) at 5 μg/mL (100 μL/well) can bind Mouse PD-L1, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. PD1-M52A2) with a linear range of 0.16-2.5 μg/mL (QC tested).

Protocol
Mouse PD-L1, Mouse IgG2a Fc Tag, low endotoxinMouse PD-L1, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. PD1-M52A2) ELISA bioactivity

Immobilized Mouse PD-L1, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. PD1-M52A2) at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse PD-1, Fc,Avitag (Cat. No. PD1-M82F4) with a linear range of 0.313-5 μg/mL (Routinely tested).

Protocol

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Price(USD) : $2100.00

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  • Number of Launched Drugs：3 Details
  • Number of Drugs in Clinical Trials：53 Details
  • Latest Research Phase：Approved
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