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Your Position: Home > MABSol® Fc Receptors > Porcine FcRn/FCGRT & B2M Heterodimer Protein

Porcine FcRn/FCGRT & B2M Heterodimer Protein

  • Synonym
    FcRn,FCGRT & B2M
  • Source
    Porcine FcRn / FCGRT & B2M (FCM-P5280) is expressed from human 293 cells (HEK293). It contains AA Asp 16 - Ser 289 (FCGRT) & Val 21 - His 118 (B2M) (Accession # Q6XAV9 (FCGRT) & Q07717 (B2M)).
    Predicted N-terminus: Asp 16 (FCGRT) & Val 21 (B2M)
  • Molecular Characterization
    FcRn(Asp 16 - Ser 289 (FCGRT) & Val 21 - His 118 (B2M)) Q6XAV9 (FCGRT) & Q07717 (B2M)

    Porcine FcRn / FCGRT & B2M heterodimer protein, produced by co-expression of FCGRT and B2M, has a calculated MW of 32.3 kDa (FCGRT) and 12.8 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus and subunit Beta-2 microglobulin (B2M) is fused with a Strep II tag at the C-terminus. The predicted N-terminus is Asp 16 (FCGRT) & Val 21 (B2M). The reducing (R) protein migrates as 36 kDa (FCGRT) and 15 kDa (B2M) respectively.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Porcine FcRn / FCGRT & B2M (Cat. No. FCM-P5280) SDS-PAGE gel

Porcine FcRn / FCGRT & B2M on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

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