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Your Position: Home > Immune Checkpoint Proteins > TIGIT > Cynomolgus / Rhesus macaque TIGIT Protein (HPLC-verified)

Cynomolgus / Rhesus macaque TIGIT Protein (HPLC-verified)

  • Synonym
    TIGIT,VSIG9,VSTM3
  • Source
    Cynomolgus / Rhesus macaque TIGIT, His Tag (TIT-C5223) is expressed from human 293 cells (HEK293). It contains AA Met 89 - Ile 208 (Accession # XP_015300911.1).
    Predicted N-terminus: Met 89
  • Molecular Characterization
    TIGIT(Met 89 - Ile 208) XP_015300911.1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 15.2 kDa. The protein migrates as 18-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-HPLC.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Cynomolgus / Rhesus macaque TIGIT, His Tag (Cat. No. TIT-C5223) SDS-PAGE gel

Cynomolgus / Rhesus macaque TIGIT, His Tag on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-HPLC
Cynomolgus / Rhesus macaque TIGIT, His Tag (Cat. No. ) HPLC images

The purity of Cynomolgus / Rhesus macaque TIGIT, His Tag (Cat. No. TIT-C5223) was greater than 95% as determined by SEC-HPLC.

Bioactivity-ELISA
Cynomolgus / Rhesus macaque TIGIT, His TagCynomolgus / Rhesus macaque TIGIT, His Tag (Cat. No. TIT-C5223) ELISA bioactivity

Immobilized Cynomolgus / Rhesus macaque TIGIT (HPLC-verified) (Cat. No. TIT-C5223) at 5 μg/mL (100 μL/well) can bind Human CD155, Fc Tag (Cat. No. CD5-H5251) with a linear range of 0.078-2.5 μg/mL (QC tested).

  • Background
    T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is also known as V-set and immunoglobulin domain-containing protein 9 (VSIG9), V-set and transmembrane domain-containing protein 3 (VSTM3),which belongs to single-pass type I membrane protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level). TIGIT binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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