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Your Position: Home > Immune Checkpoint Proteins > CTLA-4 > Mouse CTLA-4 / CD152 Protein, mouse IgG2a Fc Tag

Mouse CTLA-4 / CD152 Protein, mouse IgG2a Fc Tag, low endotoxin

  • Synonym
    CTLA4,CD152
  • Source
    Mouse CTLA-4, mouse IgG2a Fc Tag, low endotoxin (CT4-M52A3) is expressed from human 293 cells (HEK293). It contains AA Glu 36 - Phe 162 (Accession # NP_033973).
    Predicted N-terminus: Glu 36
  • Molecular Characterization
    CTLA-4(Glu 36 - Phe 162) NP_033973

    This protein carries a mouse IgG2a Fc tag at the C-terminus.

    The protein has a calculated MW of 41.1 kDa. As a result of glycosylation, the protein migrates as 50-66 kDa under reducing (R) condition, and 95-105 kDa under non-reducing (NR) condition (SDS-PAGE).

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Mouse CTLA-4, mouse IgG2a Fc Tag, low endotoxin (Cat. No. CT4-M52A3) SDS-PAGE gel

Mouse CTLA-4, mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Mouse CTLA-4, mouse IgG2a Fc Tag, low endotoxinMouse CTLA-4, mouse IgG2a Fc Tag, low endotoxin (Cat. No. CT4-M52A3) ELISA bioactivity

Immobilized Mouse B7-1, Fc Tag (Cat. No. CD0-M5259) at 0.2μg/mL (100 μL/well) can bind Mouse CTLA-4, mouse IgG2a Fc tag (Cat. No. CT4-M52A3) with a linear range of 0.1-3 ng/mL (QC tested).

Mouse CTLA-4, mouse IgG2a Fc Tag, low endotoxinMouse CTLA-4, mouse IgG2a Fc Tag, low endotoxin (Cat. No. CT4-M52A3) ELISA bioactivity

Immobilized Mouse B7-2, Fc Tag (Cat. No. CD6-M5251) at 0.2μg/mL (100 μL/well) can bind Mouse CTLA-4, mouse IgG2a Fc tag (Cat. No. CT4-M52A3) with a linear range of 0.4-3 ng/mL (Routinely tested).

  • Background
    CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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