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Your Position: Home > Immune Checkpoint Proteins > PD-1 > Mouse PD-1 / PDCD1 Protein, mouse IgG2a Fc Tag

Mouse PD-1 / PDCD1 Protein, mouse IgG2a Fc Tag, low endotoxin

  • Synonym
    PDCD1,PD1,CD279,SLEB2
  • Source
    Mouse PD-1, mouse IgG2a Fc tag (PD1-M52A4) is expressed from human 293 cells (HEK293). It contains AA Ser 21 - Gln 167 (Accession # NP_032824).
    Predicted N-terminus: Ser 21
  • Molecular Characterization
    Online(Ser 21 - Gln  167) NP_032824

    This protein carries a mouse IgG2a Fc tag at the C-terminus.

    The protein has a calculated MW of 43.7 kDa. As a result of glycosylation, the protein migrates as 55-66 kDa under reducing (R) condition, and 100-130 kDa under non-reducing (NR) condition (SDS-PAGE).

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Mouse PD-1, mouse IgG2a Fc tag (Cat. No. PD1-M52A4) SDS-PAGE gel

Mouse PD-1, mouse IgG2a Fc tag on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Mouse PD-1, mouse IgG2a Fc tagMouse PD-1, mouse IgG2a Fc tag (Cat. No. PD1-M52A4) ELISA bioactivity

Immobilized Mouse PD-L1, Fc Tag (Cat. No. PD1-M5251) at 5 μg/mL (100 μL/well) can bind Mouse PD-1, mouse IgG2a Fc tag (Cat. No. PD1-M52A4) with a linear range of 0.078-0.313 μg/mL (QC tested).

Mouse PD-1, mouse IgG2a Fc tagMouse PD-1, mouse IgG2a Fc tag (Cat. No. PD1-M52A4) ELISA bioactivity

Immobilized Mouse PD-L2, Fc Tag (Cat. No. PD2-M5254) at 5 μg/mL (100 μL/well) can bind Mouse PD-1, mouse IgG2a Fc tag (Cat. No. PD1-M52A4) with a linear range of 0.078-0.625 μg/mL (Routinely tested).

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PD-1 Products

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  • Classification:Homo sapiens
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