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Your Position: Home > Bio-Markers & CD Antigens > Human EGF R / EGFRvIII / ERBB1 Protein, His Tag

Human EGFRvIII Protein

  • Synonym
    EGFR,ERBB,ERBB1,HER1,PIG61,mENA
  • Source
    Human EGFRvIII (EGI-H52H4) is expressed from human HEK293 cells. It contains AA Leu 25 - Ser 645 (Accession # NP_005219.2) while the amino acids 30-297 are missing.
    Predicted N-terminus: Leu 25
  • Molecular Characterization
    EGF R(25-29 & 298-645) NP_005219.2

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 40.5 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human EGFRvIII, His Tag (Cat. No. EGI-H52H4) SDS-PAGE gel

Human EGFRvIII, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Human EGFRvIII, His TagHuman EGFRvIII, His Tag (Cat. No. EGI-H52H4) ELISA bioactivity

Immobilized Centuximab at 2 μg/mL (100 μL/well) can bind Human EGFRvIII, His Tag (Cat. No. EGI-H52H4) with a linear range of 0.2-5 ng/mL (QC tested).

  • Background
    The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer. The type III EGF deletion-mutant receptor (EGFRvIII) is the most common mutation and was first identified in primary human glioblastoma tumors; EGFR gene amplification is correlated with the structural rearrangement of the gene. The EGFRvIII gene has an in-frame deletion of 801 base pairs, corresponding to exons 2–7 in the mRNA, resulting in the deletion of amino acids 30-297 in the extracellular domain and the generation of a glycine at the fusion point
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $410.00

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Price(USD) : $2200.00

Inventory : In stock

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