0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > All Other Proteins > Siglec-3 > Biotinylated Human Siglec-3 / CD33 / gp67 Protein, His Tag, Avi Tag

Biotinylated Human Siglec-3 / CD33 Protein, Avi Tag (Avitag™)

  • Synonym
    CD33,SIGLEC3,gp67
  • Source
    Biotinylated Human Siglec-3 / CD33, His Tag (CD3-H82E7) is expressed from human 293 cells (HEK293). It contains AA Asp 18 - His 259 (Accession # AAH28152.1).
    Predicted N-terminus: Asp 18
  • Molecular Characterization
    Siglec-3(Asp 18 - His 259) AAH28152.1

    This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.

    The protein has a calculated MW of 29.4 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by reduced SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human Siglec-3 / CD33, His Tag (Cat. No. CD3-H82E7) SDS-PAGE gel

Biotinylated Human Siglec-3 / CD33, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity
Biotinylated Human Siglec-3 / CD33, His TagBiotinylated Human Siglec-3 / CD33, His Tag (Cat. No. CD3-H82E7) ELISA bioactivity

Immobilized anti human CD33 MAb, Mouse IgG1 (Clone # 6C5/2) at 2 μg/mL (100 μL/well) can bind Biotinylated Human Siglec-3, His Tag (Cat. No. CD3-H82E7) with a linear range of 0.078-2.5 ng/mL (QC tested).

Comments (0)

Order Details

Price(USD) : $350.00

Price(USD) : $1380.00

Promotion & Exhibitions

  • PEGS Boston 2018
  • WPC Boston 2018

Siglec-3 Products

  • Name:Myeloid cell surface antigen CD33
  • Classification:Homo sapiens
  • Number of Launched Drugs:1 Details
  • Number of Drugs in Clinical Trials:26 Details
  • Lastest Research Phase:Approved
  • MORE

Datasheet & Documentation

Contact Us

+1  800-810-0816 (US)
+86 400-682-2521 (AP)

Questions & Comments

This web search service is supported by Google Inc.

totop