Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™)
The protein has a calculated MW of 33.8 kDa. The protein migrates as 40-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 10 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human BCMA, Fc,Avitag, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human BAFF, Fc Tag, active trimer (Cat. No. BAF-H5261) at 2 μg/mL (100 μL/well) can bind Biotinylated Human BCMA, Fc,Avitag, premium grade (Cat. No. BC7-H82F0) with a linear range of 0.1-2 ng/mL (QC tested).
Immobilized Anti-Human BCMA Mab at 2 μg/mL (100 μL/well) can bind Biotinylated Human BCMA, Fc,Avitag, premium grade (Cat. No. BC7-H82F0) with a linear range of 0.1-3 ng/mL (Routinely tested).
Quantitative Analysis of Bispecific T-cell Engager (CD3 X BCMA) in human serum.
Immobilized Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free (Cat. No. CDD-H52W1) at 2 μg/mL, add increasing concentrations of Bispecific T cell Engager (CD3 X BCMA) in 10% human serum and then add Biotinylated Human BCMA, Fc,Avitag, premium grade (Cat. No. BC7-H82F0) at 0.2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 4 ng/mL (Routinely tested).
Immobilized Human APRIL, His,Flag Tag, active trimer (Cat. No. APL-H52D1) at 1 μg/mL (100 μL/well) can bind Biotinylated Human BCMA, Fc,Avitag, premium grade (Cat. No. BC7-H82F0) with a linear range of 0.1-2 ng/mL (Routinely tested).
2e5 of anti-BCMA CAR-293 cells were stained with 100 μL of 3 μg/mL of Biotinylated Human BCMA, Fc,Avitag, premium grade (Cat. No. BC7-H82F0) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (QC tested).
Human T cells were transfected with anti-BCMA CAR and cultured for 3 days. Three days post-transfection, 1e6 cells were first incubated with 50 μL Biotinylated Human BCMA, Fc,Avitag, premium grade (Cat. No. BC7-H82F0, 8 μg/mL), washed and then stained with PE Streptavidin and analyzed by flow cytometry. (Data are kindly provided by PREGENE Biopharma)
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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