>MABSol® Biotin Labeled Proteins
>Biotinylated Human HGF R / c-MET / AUTS9 Protein, His Tag, Avi Tag
This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 104.3 kDa. The protein migrates as 40-45 kDa α subunit and 75 kDa β subunit under reducing (R) condition (SDS-PAGE) due to glycosylation.
The mature form of HGFR is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
Biotinylated Human HGF R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human HGF protein (Cat. No. SB Human HGF Protein (10463-HNAS)) at 10 μg/mL (100 μL/well) can bind Biotinylated Human HGF R, His Tag (Cat. No. MET-H82E1) with a linear range of 0.04-0.31 μg/mL (QC tested).
Authors: Shiyong Gong, et al.
Journal: Mabs 2017
Application: Cell surface dual binding assay by FACS
The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.
Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $350.00
Inventory : In stock
Price(USD) : $1380.00
Inventory : In stock
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