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Your Position: Home > Receptor Tyrosine Kinases > HGF R > Biotinylated Human HGF R / c-MET / AUTS9 Protein, His Tag, Avi Tag

Biotinylated Human HGF R / c-MET Protein, Avi Tag (Avitag™)

  • Synonym
    MET,AUTS9,HGFR,RCCP2,c-Met
  • Source
    Biotinylated Human HGF R, His Tag (MET-H82E1) is expressed from human 293 cells (HEK293). It contains AA Glu 25 - Thr 932 (Accession # AAI30421.1).
    Predicted N-terminus: Glu 25 (α chain) & Ser 308 (β chain)
  • Molecular Characterization
    Online(Glu 25 - Thr 932) AAI30421.1

    This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.

    The protein has a calculated MW of 104.3 kDa. The protein migrates as 40-45 kDa α subunit and 80 kDa β subunit under reducing (R) condition (SDS-PAGE) due to glycosylation.

    The mature form of HGFR is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human HGF R, His Tag (Cat. No. MET-H82E1) SDS-PAGE gel

Biotinylated Human HGF R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Biotinylated Human HGF R, His TagBiotinylated Human HGF R, His Tag (Cat. No. MET-H82E1) ELISA bioactivity

Immobilized Human HGF protein at 10 μg/mL (100 μL/well) can bind Biotinylated Human HGF R, His Tag (Cat. No. MET-H82E1) with a linear range of 0.04-0.31 μg/mL (QC tested).

  • Citations
  • Background
    Hepatocyte growth factor receptor (HGFR) is also known as mesenchymal-epithelial transition factor (MET), c-Met, and is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. HGFR protein possesses tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. HGFR is normally expressed by cells of epithelial origin, while expression of HGF is restricted to cells of mesenchymal origin. Upon HGF stimulation, HGFR induces several biological responses that collectively give rise to a program known as invasive growth. Abnormal HGFR activation in cancer correlates with poor prognosis, where aberrantly active HGFR triggers tumor growth, formation of new blood vessels (angiogenesis) that supply the tumor with nutrients, and cancer spread to other organs (metastasis). HGFR is deregulated in many types of human malignancies, including cancers of kidney, liver, stomach, breast, and brain. Normally, only stem cells and progenitor cells express HGFR, However, cancer stem cells are thought to hijack the ability of normal stem cells to express HGFR, and thus become the cause of cancer persistence and spread to other sites in the body. Various mutations in the HGFR gene are associated with papillary renal carcinoma. HGFR mediates a complex program known as invasive growth. Activation of HGFR triggers mitogenesis, and morphogenesis.
  • References
    • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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