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Your Position: Home > ActiveMax® Cytokines > Activin A > Recombinant Human Activin A / INHBA Protein,Tag Free

ActiveMax® Recombinant Human Activin A / INHBA,low endotoxin

  • Synonym
    Activin A,INHBA
  • Source
    ActiveMax® Human Activin A,Tag Free, low endotoxin (ACA-H421b) is expressed from human 293 cells (HEK293). It contains AA Gly 311 - Ser 426 (Accession # AAH07858.1).
    Predicted N-terminus: Gly 311
  • Molecular Characterization
    Activin A(Gly 311 - Ser 426) AAH07858.1

    This protein carries no "tag".

    The protein has a calculated MW of 13 kDa. the protein migrates as 14 kDa under reducing (R) condition, and 27 kDa under non-reducing (NR) condition (SDS-PAGE).

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 0.056% TFA in 60% ACN. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
ActiveMax® Human Activin A,Tag Free, low endotoxin (Cat. No. ACA-H421b) SDS-PAGE gel

ActiveMax® Human Activin A,Tag Free, low endotoxin on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
ActiveMax® Human Activin A,Tag Free, low endotoxinActiveMax® Human Activin A,Tag Free, low endotoxin (Cat. No. ACA-H421b) ELISA bioactivity

Immobilized ActiveMax® Human Activin A,Tag Free, low endotoxin (Cat. No. ACA-H421b) at 10μg/mL (100 μL/well) can bind Human ACVR1B, Fc Tag (Cat. No. ACB-H5251) with a linear range of 0.3-2.5 μg/mL (QC tested).

ActiveMax® Human Activin A,Tag Free, low endotoxinActiveMax® Human Activin A,Tag Free, low endotoxin (Cat. No. ACA-H421b) ELISA bioactivity

Immobilized ActiveMax® Human Activin A,Tag Free, low endotoxin (Cat. No. ACA-H421b) at 2 μg/mL (100 μL/well) can bind Human ACVR2A, Fc Tag (Cat. No. ACA-H5253) with a linear range of 0.08-1.25 μg/mL (QC tested).

ActiveMax® Human Activin A,Tag Free, low endotoxinActiveMax® Human Activin A,Tag Free, low endotoxin (Cat. No. ACA-H421b) ELISA bioactivity

The bio-activity of ActiveMax® Human Activin A,Tag Free, low endotoxin (Cat. No. ACA-H421b) was determined by dose-dependent inhibition of the proliferation of MPC-11 cells. The EC50 for this effect is typically ≤ 9.5ng/ml (Routinely tested).

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