This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 87.4 kDa. GP3-H5258 may be cleaved into secrete GPC3, short propeptide (aa 483-559 predicted) & Fc tag, long propeptide (aa 359-559 predicted) & Fc tag. The protein migrates as 100 kDa (secrete GPC3 & propeptide & Fc tag), 58 kDa (long propeptide & Fc tag), and 40 kDa (short propeptide & Fc tag) on a SDS-PAGE gel under reducing (R) condition.
>90% GPC3 and 2-8% human IgG1 Fc fragment as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human Glypican 3 / GPC3, Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
293 cells were transfected with anti-GPC3-scFv and RFP tag. 2e5 of the cells were first stained with B. human Glypican 3 / GPC3 protein, Fc Tag (Cat. No. GP3-H5258, 3ug/ml) and C. human Fc tag protein control, followed by FITC-conjugated anti-human IgG Fc antibody. A. Non-transfected 293 cells and C. human Fc tag protein control were used as negative control. RFP was used to evaluate CAR (anti-GPC3-scFv) expression and FITC was used to evaluate the binding activity of human Glypican 3 / GPC3 protein, Fc Tag (Cat. No. GP3-H5258).
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Price(USD) : $300.00
Price(USD) : $1980.00
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