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Your Position: Home > All Other Proteins > Glypican 3 > Human Glypican 3 / GPC3 Protein, Fc Tag

Human Glypican 3 / GPC3 Protein, Fc Tag, low endotoxin

  • Synonym
    GPC3,OCI5,Glypican-3,GTR2-2,MXR7,DGSX,SDYS ,SGB,SGBS,SGBS1
  • Source
    Human Glypican 3 / GPC3, Fc Tag, low endotoxin (GP3-H5258) is expressed from human 293 cells (HEK293). It contains AA Gln 25 - His 559 (Accession # NP_004475).
    Predicted N-terminus: Gln25, Ser359, Val483
  • Molecular Characterization
    Glypican 3(Gln 25 - His 559) NP_004475

    This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 87.4 kDa. GP3-H5258 may be cleaved into secrete GPC3, short propeptide (aa 483-559 predicted) & Fc tag, long propeptide (aa 359-559 predicted) & Fc tag. The protein migrates as 100 kDa (secrete GPC3 & propeptide & Fc tag), 58 kDa (long propeptide & Fc tag), and 40 kDa (short propeptide & Fc tag) on a SDS-PAGE gel under reducing (R) condition.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >90% GPC3 and 2-8% human IgG1 Fc fragment as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Human Glypican 3 / GPC3, Fc Tag, low endotoxin (Cat. No. GP3-H5258) SDS-PAGE gel

Human Glypican 3 / GPC3, Fc Tag, low endotoxin on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity
Human Glypican 3 / GPC3, Fc Tag, low endotoxinHuman Glypican 3 / GPC3, Fc Tag, low endotoxin (Cat. No. GP3-H5258) ELISA bioactivity

Immobilized Human FGF basic, Tag Free (Cat. No. BFF-H4117) at 2μg/mL (100 μL/well) can bind Human Glypican 3, Fc Tag (Cat. No. GP3-H5258) with a linear range of 0.48-7.8 ng/mL (QC tested).

Human Glypican 3 / GPC3, Fc Tag, low endotoxinHuman Glypican 3 / GPC3, Fc Tag, low endotoxin (Cat. No. GP3-H5258) ELISA bioactivity

Immobilized Human Glypican 3, Fc Tag (Cat. No. GP3-H5258) at 0.5 μg/mL (100 μL/well) can bind Anti-Human GPC3 Mab with a linear range of 0.8-6 ng/mL (Routinely tested).

Evaluation of CAR expression
FACS Analysis of Anti-GPC3 CAR Expression
Evaluation of CAR expression

293 cells were transfected with anti-GPC3-scFv and RFP tag. 2e5 of the cells were first stained with B. human Glypican 3 / GPC3 protein, Fc Tag (Cat. No. GP3-H5258, 3ug/ml) and C. human Fc tag protein control, followed by FITC-conjugated anti-human IgG Fc antibody. A. Non-transfected 293 cells and C. human Fc tag protein control were used as negative control. RFP was used to evaluate CAR (anti-GPC3-scFv) expression and FITC was used to evaluate the binding activity of human Glypican 3 / GPC3 protein, Fc Tag (Cat. No. GP3-H5258).

  • Background
    Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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