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Your Position: Home > All Other Proteins > Integrin alpha V beta 5 > Human Integrin alpha V beta 5 / ITGAV&ITGB5 / ITGAVB5 Heterodimer Protein

Human Integrin alpha V beta 5 (ITGAV&ITGB5) Heterodimer Protein

  • Synonym
    Integrin alpha V beta 5,ITGAV&ITGB5
  • Source
    Human ITGAV&ITGB5, His Tag (IT5-H52W5) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Val 992 (ITGAV) & Gly 24 - Asn 719 (ITGB5) (Accession # NP_002201 (ITGAV) & AAH06541 (ITGB5)).
    Predicted N-terminus: Phe 31 (ITGAV) & Gly 24 (ITGB5)
  • Molecular Characterization
    Online(Phe 31 - Val 992 (ITGAV) & Gly 24 - Asn 719 (ITGB5)) NP_002201 (ITGAV) & AAH06541 (ITGB5)

    Human ITGAV & ITGB5 Heterodimer Protein, produced by co-expression of ITGAV and ITGB5, has a calculated MW of 113.0 kDa (ITGAV) and 81.9 kDa (ITGB5). Subunit ITGAV is fused with polyhistidine tag at the C-terminus and followed by a acidic tail and subunit ITGB5 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 130-150 kDa (ITGAV) and 85-95 kDa (ITGB5) respectively due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human ITGAV&ITGB5, His Tag (Cat. No. IT5-H52W5) SDS-PAGE gel

Human ITGAV&ITGB5, His Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human ITGAV&ITGB5, His TagHuman ITGAV&ITGB5, His Tag (Cat. No. IT5-H52W5) ELISA bioactivity

Immobilized Vitronectin at 2 μg/mL (100 μL/well) can bind Human ITGAV&ITGB5, His Tag (Cat. No. IT5-H52W5) with a linear range of 2-20 ng/mL (QC tested).

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Price(USD) : $410.00

Price(USD) : $2880.00

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