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Your Position: Home > All Other Proteins > Integrin alpha V beta 8 > Human Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein

Human Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein

  • Synonym
    Integrin alpha V beta 8,ITGAV&ITGB8
  • Source
    Human ITGAV & ITGB8 Heterodimer Protein (IT8-H52W4) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Val 992 (ITGAV) & Glu 43 - Arg 684 (ITGB8) (Accession # NP_002201 (ITGAV) & NP_002205 (ITGB8)).
    Predicted N-terminus: Phe 31 (ITGAV) & Glu 43 (ITGB8)
  • Molecular Characterization
    Online(Phe 31 - Val 992 (ITGAV) & Glu 43 - Arg 684 (ITGB8)) NP_002201 (ITGAV) & NP_002205 (ITGB8)

    Human ITGAV & ITGB8 Heterodimer Protein, produced by co-expression of ITGAV and ITGB8, has a calculated MW of 113.0 kDa (ITGAV) and 76.5 kDa (ITGB8). Subunit ITGAV is fused with polyhistidine tag at the C-terminus and followed by a acidic tail and subunit ITGB8 contains no tag but a basic tail at the C-terminus. The reducing (R) protein migrates as 145 kDa (ITGAV) and 100-116 kDa (ITGB8) respectively due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, 2 mM MgCl2, pH8.0. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human ITGAV & ITGB8 Heterodimer Protein (Cat. No. IT8-H52W4) SDS-PAGE gel

Human ITGAV & ITGB8 Heterodimer Protein on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human ITGAV & ITGB8 Heterodimer ProteinHuman ITGAV & ITGB8 Heterodimer Protein (Cat. No. IT8-H52W4) ELISA bioactivity

Immobilized Human ITGAV & ITGB8 Heterodimer Protein (Cat. No. IT8-H52W4) at 5 μg/mL (100 μL/well) can bind Biotinylated Human TGF-Beta 1, His Tag, Avi Tag with a linear range of 0.8-13 ng/mL (QC tested).

Human ITGAV & ITGB8 Heterodimer ProteinHuman ITGAV & ITGB8 Heterodimer Protein (Cat. No. IT8-H52W4) ELISA bioactivity

Immobilized Human Fibronectin at 10 μg/mL (100 μL/well) can bind Human ITGAV & ITGB8 Heterodimer Protein (Cat. No. IT8-H52W4) with a linear range of 0.313-10 μg/mL (Routinely tested).

Human ITGAV & ITGB8 Heterodimer ProteinHuman ITGAV & ITGB8 Heterodimer Protein (Cat. No. IT8-H52W4) ELISA bioactivity

Immobilized Human Vitronectin at 5 μg/mL (100 μL/well) can bind Human ITGAV & ITGB8 Heterodimer Protein (Cat. No. IT8-H52W4) with a linear range of 0.313-10 μg/mL (Routinely tested).

  • Background
    Integrin alpha V beta 8 (ITGAV & ITGB8 or ITGAVB8) is expressed in yolk sac, placenta, brain perivascular astrocytes, Schwann cells, renal glomerular mesangial cells and pulmonary epithelial cells. Unlike other alpha V integrins, ITGAVB8 does not appear to assume different activation states, and the cytoplasmic tail does not connect to the cytoskeleton. It binds ligands containing an RGD motif, including vitronectin, fibrin and the latency associated peptide (LAP) of the latent TGF-beta complex. High affinity binding of alpha V beta 8 to LAP allows proteolytic cleavage by MT1-MMP, which releases active TGF-beta. This mechanism differs from that of alpha V beta 6, the other alpha V integrin which can activate TGF-beta from latency through non-proteolytic mechanisms. Downstream effects of TGF-beta activation include control of cell growth and associated vascularization.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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