>Bio-Markers & CD Antigens
>Integrin alpha V beta 8
>Human Integrin alpha V beta 8 / ITGAV&ITGB8 / ITGAVB8 Heterodimer Protein
Human ITGAV & ITGB8 Heterodimer Protein, produced by co-expression of ITGAV and ITGB8, has a calculated MW of 113 kDa (ITGAV) and 76.5 kDa (ITGB8). Subunit ITGAV is fused with polyhistidine tag at the C-terminus and followed by a acidic tail and subunit ITGB8 contains no tag but a basic tail at the C-terminus. The reducing (R) protein migrates as 145 kDa (ITGAV) and 100-116 kDa (ITGB8) respectively due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, 2 mM MgCl2, pH8.0. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
Human ITGAV & ITGB8 Heterodimer Protein on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human ITGAV&ITGB8 (Cat. No. IT8-H52W4) at 5μg/mL (100 μL/well) can bind biotinylated Human TGF-Beta 1 / TGFB1, His Tag & Avi Tag with a linear range of 0.8-13 ng/mL (QC tested).
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