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Your Position: Home > Immune Checkpoint Proteins > CD28 > CD8-H82F2

Biotinylated Human / Cynomolgus / Rhesus macaque CD28 Protein, Fc,Avitag™

  • Synonym
    CD28,Tp44
  • Source
    Biotinylated Human / Cynomolgus / Rhesus macaque CD28, Fc,Avitag (CD8-H82F2) is expressed from human 293 cells (HEK293). It contains AA Asn 19 - Pro 152 (Accession # AAH93698). In the region Asn 19 - Pro 152, the AA sequence of Human, Cynomolgus and Rhesus macaque CD28 are homologus.
    Predicted N-terminus: Asn 19
  • Molecular Characterization
    Online(Asn 19 - Pro 152) AAH93698

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).

    The protein has a calculated MW of 43.6 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human / Cynomolgus / Rhesus macaque CD28, Fc,Avitag (Cat. No. CD8-H82F2) SDS-PAGE gel

Biotinylated Human / Cynomolgus / Rhesus macaque CD28, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Human / Cynomolgus / Rhesus macaque CD28, Fc,AvitagBiotinylated Human / Cynomolgus / Rhesus macaque CD28, Fc,Avitag (Cat. No. CD8-H82F2) ELISA bioactivity

Immobilized Biotinylated Human / Cynomolgus / Rhesus macaque CD28, Fc,Avitag (Cat. No. CD8-H82F2) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Human B7-1, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. B71-H52A4) with a linear range of 5-78 ng/mL (QC tested).

Biotinylated Human / Cynomolgus / Rhesus macaque CD28, Fc,AvitagBiotinylated Human / Cynomolgus / Rhesus macaque CD28, Fc,Avitag (Cat. No. CD8-H82F2) ELISA bioactivity

Immobilized Anti-CD28 MAb, Mouse IgG1 at 5 μg/mL (100 μL/well) can bind Biotinylated Human / Cynomolgus / Rhesus macaque CD28, Fc,Avitag (Cat. No. CD8-H82F2) with a linear range of 2.4-39 ng/mL (Routinely tested).

Biotinylated Human / Cynomolgus / Rhesus macaque CD28, Fc,AvitagBiotinylated Human / Cynomolgus / Rhesus macaque CD28, Fc,Avitag (Cat. No. CD8-H82F2) ELISA bioactivity

Immobilized Human B7-1, Fc Tag (Cat. No. B71-H5259) at 5 μg/mL (100 μL/well) can bind Biotinylated Human / Cynomolgus / Rhesus macaque CD28, Fc,Avitag (Cat. No. CD8-H82F2) with a linear range of 0.313-5 μg/mL (Routinely tested).

  • Citations
  • Background
    T-cell-specific surface glycoprotein CD28 is also known as TP44, is a single-pass type I membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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