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Your Position: Home > MABSol® Biotin Labeled Proteins > IL23A & IL12B > Biotinylated Human IL23A & IL12B Heterodimer Protein, His Tag, Avi Tag

Biotinylated Human IL-23 alpha & IL-12 beta, Avi Tag (Avitag™)

  • Synonym
    IL-23 alpha & IL-12 beta
  • Source
    Biotinylated Human IL-23A & IL-12B Heterodimer (ILB-H82W6) is expressed from human 293 cells (HEK293). It contains AA Arg 20 - Pro 189 (IL23A) & Ile 23 - Ser 328 (IL12B) (Accession # NP_057668 (IL23A) & AAH67499 (IL12B)).
    Predicted N-terminus: His (IL23A) & Ile 23 (IL12B)
  • Molecular Characterization
    IL23A & IL12B(Arg 20 - Pro 189 (IL23A) & Ile 23 - Ser 328 (IL12B)) NP_057668 (IL23A) & AAH67499 (IL12B)

    Biotinylated Human IL23A & IL12B Heterodimer Protein, produced by co-expression of IL23A and IL12B, has a calculated MW of 21.9 kDa (IL23A) & 34.7 kDa (IL12B). Subunit IL23A is fused with an Avi tag (Avitag™), followed by a polyhistidine tag at the N-terminus and subunit IL12B contains no tag. The predicted N-terminus is His (IL23A) & Ile 23 (IL12B). The reducing (R) protein migrates as 26 kDa (IL23A) & 40-45 kDa (IL12B) respectively due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human IL-23A & IL-12B Heterodimer (Cat. No. ILB-H82W6) SDS-PAGE gel

Biotinylated Human IL-23A & IL-12B Heterodimer on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Biotinylated Human IL-23A & IL-12B HeterodimerBiotinylated Human IL-23A & IL-12B Heterodimer (Cat. No. ILB-H82W6) ELISA bioactivity

Immobilized Anti-p40 subunit of IL23(Anti-IL12B) MAb, Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human IL23A & IL12B, His Tag (Cat. No. ILB-H82W6) with a linear range of 4.9-39 ng/mL (Routinely tested).

Biotinylated Human IL-23A & IL-12B HeterodimerBiotinylated Human IL-23A & IL-12B Heterodimer (Cat. No. ILB-H82W6) ELISA bioactivity

Immobilized Anti-p19 subunit of IL23 (Anti-IL23A) MAb, Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human IL23A & IL12B, His Tag (Cat. No. ILB-H82W6) with a linear range of 1.2-19.5 ng/mL (QC tested).

Human FcRn Heterodimer Protein Cell_Base

Biotinylated Human IL-23 alpha & IL-12 beta, Avi Tag (Cat. No. ILB-H82W6) stimulates secretion of IL-17 by mousespleen cells. The ED50 for this effect is 0.191-0.2173ng/ml (Routinely tested).

  • Background
    Interleukin-23 subunit alpha (IL-23 alpha) can associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
  • References
  • Limited Use License

    The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

    Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $1380.00

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