>IL23A & IL12B
>Biotinylated Human IL23A & IL12B Heterodimer Protein, His Tag, Avi Tag
Biotinylated Human IL23A & IL12B Heterodimer Protein, produced by co-expression of IL23A and IL12B, has a calculated MW of 21.9 kDa (IL23A) & 34.7 kDa (IL12B). Subunit IL23A is fused with an Avi tag (Avitag™), followed by a polyhistidine tag at the N-terminus and subunit IL12B contains no tag. The predicted N-terminus is His (IL23A) & Ile 23 (IL12B). The reducing (R) protein migrates as 26 kDa (IL23A) & 40-45 kDa (IL12B) respectively due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
Biotinylated Human IL23A & IL12B, His Tag, Avi Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Anti-p40 subunit of IL23（Anti-IL12B） MAb, Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human IL23A & IL12B, His Tag (Cat. No. ILB-H82W6) with a linear range of 4.9-39 ng/mL (Routinely tested).
Captured Biotinylated Human IL23A & IL12B, His Tag, Avi Tag (Cat. No. ILB-H82W6) on Biotin CAP - Series S sensor Chip can bind Human IL-23 R, His Tag (Cat. No. ILR-H52H4) affinity constant of 4.77 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Biotinylated Human IL-23 alpha & IL-12 beta, Avi Tag (Cat. No. ILB-H82W6) stimulates secretion of IL-17 by mousespleen cells. The ED50 for this effect is 0.191-0.2173ng/ml (Routinely tested).
Cell based assay shows that the secretion of IL-17 induced by Biotinylated Human IL-23 alpha & IL-12 beta Heterodimer Protein (Cat. No. ILB-H82W6) is inhibited by increasing concentration of the anti-human IL-23 neutralizing antibody. The IC50 is between 0.25-1.40 ng/mL (Routinely tested).
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Price(USD) : $350.00
Price(USD) : $1380.00
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