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Your Position: Home > MABSol® Biotin Labeled Proteins > Biotinylated Human IL-7 R alpha / CD127 / IL7Ra Protein, Fc Tag, Avi Tag

Biotinylated Human IL-7 R alpha / CD127 Protein, Fc Tag, Avi Tag (Avitag™)

  • Synonym
    IL7Ra,CD127
  • Source
    Biotinylated Human IL-7 RA / CD127, Fc Tag (IL7-H82F8) is expressed from human 293 cells (HEK293). It contains AA Glu 21 - Gly 236 (Accession # P16871).
    Predicted N-terminus: Glu 21
  • Molecular Characterization
    IL-7 R alpha(Glu 21 - Gly 236) P16871

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).

    The protein has a calculated MW of 53.3 kDa. As a result of glycosylation, the protein migrates as 70-100 kDa under reducing (R) condition, and 130-200 kDa under non-reducing (NR) condition (SDS-PAGE).

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human IL-7 RA / CD127, Fc Tag (Cat. No. IL7-H82F8) SDS-PAGE gel

Biotinylated Human IL-7 RA / CD127, Fc Tag on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Biotinylated Human IL-7 RA / CD127, Fc TagBiotinylated Human IL-7 RA / CD127, Fc Tag (Cat. No. IL7-H82F8) ELISA bioactivity

Immobilized Human IL-7, Tag Free (Cat. No. IL7-H4219) at 10 μg/mL (100 μL/well) can bind Biotinylated Human IL-7 R alpha, Fc Tag (Cat. No. IL7-H82F8) with a linear range of 0.02-0.15 μg/mL (QC tested).

  • Background
    Interleukin-7 receptor subunit alpha (IL-7 RA or IL7Ra) is also known as cluster of differentiation 127 (CD127). IL-7 RA is a type I cytokine receptor and is a subunit of the functional Interleukin-7 receptor and thymic stromal lymphopoietin (TSLP) receptors. IL-7 RA has been shown to play a critical role in the development of immune cells called lymphocytes - specifically in a process known as V(D)J recombination[citation needed]. This protein is also found to control the accessibility of a region of the genome that contains the T-cell receptor gamma gene, by STAT5 and histone acetylation.
  • References
  • Limited Use License

    The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

    Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Order Details

Price(USD) : $350.00

Inventory : In stock

Price(USD) : $1380.00

Inventory : In stock

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