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Your Position: Home > Immune Checkpoint Proteins > CD47 > Unconjugated Human CD47 Protein, His Tag & Avi Tag

Unconjugated Human CD47 Protein, His Tag & Avi Tag

  • Synonym
    CD47,MER6,IAP,OA3
  • Source
    Unconjugated Human CD47, His Tag & Avi Tag (CD7-HA2E9) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Pro 139 (Accession # NP_942088).
    Predicted N-terminus: Gln 19
  • Molecular Characterization
    CD47(Gln 19 - Pro 139) NP_942088

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

    The protein has a calculated MW of 16.9 kDa. The protein migrates as 33-44 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Application
    CD7-HA2E9 is the unconjugated version of CD7-H82E9 (Biotinylated Human CD47, His Tag). It can be utilized for assay development or customized biotinylation according to the experimental designs.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 10 mM Bicine, pH8.3. Normally trehalose is added as protectant before lyophilization.

    Appropriate buffer for directly in vitro enzymatical labeling, please contact us for customized formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Unconjugated Human CD47, His Tag & Avi Tag (Cat. No. CD7-HA2E9) SDS-PAGE gel

Unconjugated Human CD47, His Tag & Avi Tag on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Unconjugated Human CD47, His Tag & Avi TagUnconjugated Human CD47, His Tag & Avi Tag (Cat. No. CD7-HA2E9) ELISA bioactivity

Immobilized Human SIRP alpha, Fc Tag (Cat. No. SIA-H5251) at 2μg/mL (100 μL/well) can bind Unconjugated Human CD47, His Tag, Avi Tag (Cat. No. CD7-HA2E9) with a linear range of 2-12.8 ng/mL (QC tested).

Unconjugated Human CD47, His Tag & Avi TagUnconjugated Human CD47, His Tag & Avi Tag (Cat. No. CD7-HA2E9) ELISA bioactivity

Immobilized Anti-CD47 MAb, Human IgG4 at 2μg/mL (100 μL/well) can bind Unconjugated Human CD47, His Tag, Avi Tag (Cat. No. CD7-HA2E9) with a linear range of 0.05-2.05 ng/mL (QC tested).

  • Background
    Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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