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Your Position: Home > Receptor Tyrosine Kinases > VEGF R3 > Human VEGF R3 / FLT4 Protein, Fc Tag

Human VEGF R3 / FLT4 Protein, Fc Tag

  • Synonym
    Flt-4,FLT4,LMPH1A,PCLFLT41,VEGFR3,VEGFR-3,FLT-4,FLT-41,FLT41,PCL
  • Source
    Human VEGF R3, Fc Tag (FL4-H5251) is expressed from human 293 cells (HEK293). It contains AA Tyr 25 - Ile 776 (Accession # NP_002011.2).
    Predicted N-terminus: Tyr 25
  • Molecular Characterization
    VEGF R3(Tyr  25 - Ile 776) NP_002011.2

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 111.2 kDa. As a result of glycosylation, the protein migrates as 140-160 kDa under reducing (R) condition, and 280-320 kDa under non-reducing (NR) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human VEGF R3, Fc Tag (Cat. No. FL4-H5251) SDS-PAGE gel

Human VEGF R3, Fc Tag on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Human VEGF R3, Fc TagHuman VEGF R3, Fc Tag (Cat. No. FL4-H5251) ELISA bioactivity

Immobilized Human VEGF-C, His Tag (Cat. No. VEC-H4225) at 5μg/mL (100 μL/well) can bind Human VEGF R3, Fc Tag (Cat. No. FL4-H5251) with a linear range of 0.6-10 ng/mL (QC tested).

Human VEGF R3, Fc TagHuman VEGF R3, Fc Tag (Cat. No. FL4-H5251) ELISA bioactivity

Immobilized Human VEGF R3, Fc Tag (Cat. No. FL4-H5251) at 5μg/mL (100 μL/well) can bind Human VEGF-D, His Tag (Cat. No. VED-H5228) with a linear range of 0.08-0.6 μg/mL (Routinely tested).

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