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Your Position: Home > Bio-Markers & CD Antigens > CD58 > Human CD58 Protein, Fc Tag

Human CD58 / LFA-3 Protein, Fc Tag

  • Synonym
    CD58,LFA3,Ag3
  • Source
    Human CD58, Fc Tag (LF3-H5256) is expressed from human 293 cells (HEK293). It contains AA Phe 29 - Arg 215 (Accession # AAH05930).
    Predicted N-terminus: Phe 29
  • Molecular Characterization
    CD58(Phe 29  - Arg 215) AAH05930

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 48.1 kDa. As a result of differtent glycosylation, the protein migrates as 60-75 kDa under reducing (R) condition, and 125-150 kDa under non-reducing (NR) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human CD58, Fc Tag (Cat. No. LF3-H5256) SDS-PAGE gel

Human CD58, Fc Tag on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Human CD58, Fc TagHuman CD58, Fc Tag (Cat. No. LF3-H5256) ELISA bioactivity

Immobilized Human CD2, His Tag (Cat. No. CD2-H5226) at 2μg/mL (100 μL/well) can bind Human CD58, Fc Tag (Cat. No. LF3-H5256) with a linear range of 10-156 ng/mL (QC tested).

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