0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > Immune Checkpoint Proteins > LAG-3 > Human LAG3 / CD223 Protein, Fc Tag

Human LAG3 / CD223 Protein, Fc Tag (HPLC-verified)

  • Synonym
    LAG3,CD223,FDC
  • Source
    Human LAG-3, Fc Tag (HPLC-verified) (LA3-H5255) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Leu 450 (Accession # P18627-1).
    Predicted N-terminus: Leu 23
  • Molecular Characterization
    LAG-3(Leu 23 - Leu 450) P18627-1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 72.8 kDa. The protein migrates as 80-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-HPLC.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Human LAG-3, Fc Tag (HPLC-verified) (Cat. No. LA3-H5255) SDS-PAGE gel

Human LAG-3, Fc Tag (HPLC-verified) on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-HPLC
Human LAG-3, Fc Tag (HPLC-verified) (Cat. No. ) HPLC images

Human LAG-3, Fc Tag (HPLC-verified) (Cat. No. LA3-H5255) was greater than 90% as determined by SEC-HPLC.

Bioactivity-ELISA
Human LAG-3, Fc Tag (HPLC-verified)Human LAG-3, Fc Tag (HPLC-verified) (Cat. No. LA3-H5255) ELISA bioactivity

Immobilized Human LAG-3, Fc Tag (HPLC-verified) (Cat. No. LA3-H5255) at 2 μg/mL (100 μL/well) can bind Madarex LAG-3 MAb with a linear range of 0.4-3 ng/mL (QC tested).

Comments (0)

Order Details

Price(USD) : $300.00

Price(USD) : $2100.00

Promotion & Exhibitions

  • PEGS Boston 2018
  • WPC Boston 2018

Datasheet & Documentation

Contact Us

+1  800-810-0816 (US)
+86 400-682-2521 (AP)

Questions & Comments

This web search service is supported by Google Inc.

totop