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Your Position: Home > Immune Checkpoint Proteins > 2B4 > Biotinylated Human 2B4 / CD244 / SLAMF4 Protein, Fc,Avitag

Biotinylated Human 2B4 / CD244 / SLAMF4 Protein, Fc,Avitag™

  • Synonym
    CD244,2B4,SLAMF4,NKR2B4,NAIL,h2B4
  • Source
    Biotinylated Human 2B4, Fc,Avitag (2B4-H82F0) is expressed from human 293 cells (HEK293). It contains AA Cys 22 - Arg 221 (Accession # NP_057466.1).
    Predicted N-terminus: Cys 22
  • Molecular Characterization
    Online(Cys 22 - Arg 221) NP_057466.1

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).

    The protein has a calculated MW of 50.7 kDa. As a result of glycosylation, the protein migrates as 65-80 kDa under reducing (R) condition, and 130-160 kDa under non-reducing (NR) condition (SDS-PAGE).

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human 2B4, Fc,Avitag (Cat. No. 2B4-H82F0) SDS-PAGE gel

Biotinylated Human 2B4, Fc,Avitag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Human 2B4, Fc,AvitagBiotinylated Human 2B4, Fc,Avitag (Cat. No. 2B4-H82F0) ELISA bioactivity

Immobilized Human CD48, Fc tag at 10 μg/mL (100 μL/well) can bind Biotinylated Human 2B4, Fc,Avitag (Cat. No. 2B4-H82F0) with a linear range of 0.01-0.156 μg/mL (QC tested).

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Price(USD) : $350.00

Price(USD) : $1380.00

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