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Your Position: Home > Immune Checkpoint Proteins > LILRB2 > CDD-H5259

Human LILRB2 / CD85d / ILT4 Protein, Fc Tag (MALS verified)

  • Synonym
    LILRB2,ILT4,LIR2,MIR10,CD85d
  • Source
    Human LILRB2, Fc Tag (CDD-H5259) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Val 461 (Accession # AAH36827).
    Predicted N-terminus: Gln 22
  • Molecular Characterization
    Online(Gln 22 - Val 461) AAH36827

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 74.4 kDa. The protein migrates as 90-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human LILRB2, Fc Tag (Cat. No. CDD-H5259) SDS-PAGE gel

Human LILRB2, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Human LILRB2, Fc Tag (Cat. No. ) MALS images

The purity of Human LILRB2, Fc Tag (Cat. No. CDD-H5259) is more than 95% and the molecular weight of this protein is around 160-200 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human LILRB2, Fc TagHuman LILRB2, Fc Tag (Cat. No. CDD-H5259) ELISA bioactivity

Immobilized Human LILRB2, Fc Tag (Cat. No. CDD-H5259) at 1 μg/mL (100 μL/well) can bind Anti-LILRB2 Antibody, Human IgG1 with a linear range of 1-2 ng/mL (QC tested).

Human LILRB2, Fc TagHuman LILRB2, Fc Tag (Cat. No. CDD-H5259) ELISA bioactivity

Immobilized Human HLA-G RIIPRHLQL Tetramer Protein (Cat. No. HLG-H52E9) at 5 μg/mL (100 μL/well) can bind Human LILRB2, Fc Tag (Cat. No. CDD-H5259) with a linear range of 0.01-0.313 μg/mL (Routinely tested).

Human LILRB2, Fc TagHuman LILRB2, Fc Tag (Cat. No. CDD-H5259) ELISA bioactivity

Serial dilutions of Anti-LILRB2 Antibody, Human IgG1 were added into Human LILRB2, Fc Tag (Cat. No. CDD-H5259): Human HLA-G RIIPRHLQL Tetramer Protein (Cat. No. HLG-H52E9) binding reactions. The half maximal inhibitory concentration (IC50) is 0.2403 μg/mL (Routinely tested).

  • Background
    Leukocyte immunoglobulin-like receptor subfamily B member 2 (LILRB2) is also known as CD85 antigen-like family member D (CD85d), Immunoglobulin-like transcript 4 (ILT-4), Monocyte / macrophage immunoglobulin-like receptor 10 (MIR-10), which is a member of the the subfamily B class of LIR receptors. LILRB2 is receptor for class I MHC antigens. LILRB2 recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. LILRB2 competes with CD8A for binding to class I MHC antigens. LILRB2 / CD85d inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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