This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 19.0 kDa. The protein migrates as 23 kDa and 24 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
Human CD40 Ligand, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Flow Cytometry assay shows that Human CD40 Ligand, His Tag (Cat. No. CDL-H5248) can bind to 293T cell overexpressing human CD40. The concentration of Human CD40 Ligand used is 0.1 μg/ml (Routinely tested).
FACS analysis shows that the binding of Human CD40 Ligand, His Tag (Cat. No. CDL-H5248) to 293T cell overexpressing human CD40 was inhibited by increasing concentration of neutralizing Anti-human CD40 antibody. The concentration of Human CD40 Ligand used is 0.1 μg/ml. The IC50 is 1.181 μg/ml (Routinely tested).
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Price(USD) : $298.00
Price(USD) : $1980.00
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