Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free is produced by co-expression of CD3E and CD3D, has a calculated MW of 16.7 kDa (CD3E), 13.5 kDa (CD3D) . Subunit CD3E is fused with a polyhistidine tag at the C-terminus and subunit CD3D contains no tag. The predicted N-terminus is Asp 23 (CD3E) & Phe 22 (CD3D). The reducing (R) heterodimer protein migrates as 24-32 kDa (CD3E) and 22 kDa (CD3D) due to glycosylation.
>90% as determined by SDS-PAGE.
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Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Mouse Anti-Human CD3 (SP34-2) at 5 μg/mL (100 μL/well) can bind Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free (Cat. No. CDD-H52W4) with a linear range of 0.3-5 ng/mL (QC tested).
Authors: Ostrand-rosenberg, Suzanne ,et al.
Journal: US20180291114A1 43384
Authors: Horn LA, et al.
Journal: Oncotarget 2017
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