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>CD3E & CD3D
>Human CD3 epsilon & CD3 delta Heterodimer Protein, His Tag&Tag Free
Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free is produced by co-expression of CD3E and CD3D, has a calculated MW of 16.7 kDa (CD3E), 13.5 kDa (CD3D) . Subunit CD3E is fused with a polyhistidine tag at the C-terminus and subunit CD3D contains no tag. The predicted N-terminus is Asp 23 (CD3E) & Phe 22 (CD3D). The reducing (R) heterodimer protein migrates as 24-32 kDa (CD3E) and 22 kDa (CD3D) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Mouse Anti-Human CD3 (SP34-2) at 5 μg/mL (100 μL/well) can bind Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free (Cat. No. CDD-H52W4) with a linear range of 0.3-5 ng/mL (QC tested).
Authors: Ostrand-rosenberg, Suzanne ,et al.
Journal: US20180291114A1 43384
Authors: Horn LA, et al.
Journal: Oncotarget 2017
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