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Your Position: Home > All Other Proteins > CD3E & CD3D > Human CD3E & CD3D Heterodimer Protein, His Tag

Human CD3 epsilon & CD3 delta Heterodimer Protein

  • Synonym
    CD3E & CD3D,CD3 delta & CD3 epsilon,CD3 delta / epsilon
  • Source
    Human CD3 epsilon & CD3 delta Heterodimer Protein, Asp 23 - Asp 126 (CD3E, P07766) and & Phe 22 - Ala 105 (CD3D, P04234), was produced in human 293 cells (HEK293) at ACRObiosystems.
    Predicted N-terminus: Asp 23 (CD3E) & Phe 22 (CD3D)
  • Molecular Characterization
    CD3E & CD3D(Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D)) P07766 (CD3E) & P04234 (CD3D)

    Human CD3 epsilon & CD3 delta Heterodimer Protein is produced by co-expression of CD3E and CD3D, has a calculated MW of 16.7 kDa (CD3E), 13.5 kDa (CD3D) . Subunit CD3E is fused with a polyhistidine tag at the C-terminus and subunit CD3D contains no tag. The predicted N-terminus is Asp 23 (CD3E) & Phe 22 (CD3D). The reducing (R) heterodimer protein migrates as 24-32 kDa (CD3E) and 22 kDa (CD3D) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human CD3E & CD3D, His Tag (Cat. No. CDD-H52W4) SDS-PAGE gel

Human CD3E & CD3D, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity
Human CD3E & CD3D, His TagHuman CD3E & CD3D, His Tag (Cat. No. CDD-H52W4) ELISA bioactivity

Immobilized OKT3 MAb at 5 μg/mL (100 μL/well) can bind Human CD3E & CD3D, His Tag (Cat. No. CDD-H52W4) with a linear range of 0.625-5 μg/mL (QC tested).

Human CD3E & CD3D, His TagHuman CD3E & CD3D, His Tag (Cat. No. CDD-H52W4) ELISA bioactivity

Immobilized SP34-2 at 5 μg/mL (100 μL/well) can bind Human CD3E & CD3D, His Tag (Cat. No. CDD-H52W4) with a linear range of 0.3-5 ng/mL (Routinely tested).

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