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Your Position: Home > All Other Proteins > Integrin alpha 6 beta 1 > Human Integrin alpha 6 beta 1 (ITGA6&ITGB1) Heterodimer Protein

Human Integrin alpha 6 beta 1 (ITGA6&ITGB1) Heterodimer Protein

  • Synonym
    Integrin alpha 6 beta 1,ITGA6B1,ITGA6&ITGB1,CD49f,VLA-6
  • Source
    Recombinant Human Integrin alpha 6 beta 1 (ITGA6&ITGB1) Heterodimer Protein (Human ITGA6 & ITGB1) Phe 24 - Gly 1012 (Integrin alpha 6, P23229-2) and Gln 21 - Asp 728 (Integrin beta 1, P05556-1) was co-expressed in human 293 cells (HEK293) at ACRObiosystems.
    Predicted N-terminus: Phe 24 (ITGA6) & Gln 21 (ITGB1)
  • Molecular Characterization
    ITGA6B1(Phe 24 - Gly 1012 (ITGA6) & Gln 21 - Asp 728 (ITGB1)) P23229-2 (ITGA6) & P05556-1 (ITGB1)

    Human ITGA6 & ITGB1 Heterodimer Protein, produced by co-expression of ITGA6 and ITGB1, has a calculated MW of 116.8 kDa (ITGA6) and 83.7 kDa (ITGB1). Subunit ITGA6 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The predicted N-terminus is Phe 24 (ITGA6) & Gln 21 (ITGB1). As a result of glycosylation, the protein migrates as 120-130 kDa under reducing (R) condition, and 100-125 kDa and 130-150 kDa under non-reducing (NR) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human ITGA6 & ITGB1 Heterodimer Protein (Cat. No. IT1-H52W7) SDS-PAGE gel

Human ITGA6 & ITGB1 Heterodimer Protein on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

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