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Your Position: Home > Recombinant Proteins > Recombinant Human EGF R / ERBB1 / HER1 Protein

Human EGF R Protein

  • Synonym
    EGFR,ERBB,ERBB1,HER1,PIG61,mENA
  • Source
    Human EGF R, His Tag (EGR-H5222) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645 (Accession # P00533-1).
    Predicted N-terminus: Leu 25
  • Molecular Characterization
    EGF R(Leu 25 - Ser 645) P00533-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 70.5kDa. The protein migrates as 110-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human EGF R, His Tag (Cat. No. EGR-H5222) SDS-PAGE gel

Human EGF R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Human EGF R, His TagHuman EGF R, His Tag (Cat. No. EGR-H5222) ELISA bioactivity

Immobilized Cetuximab at 2 μg/mL (100 μL/well) can bind Human EGF R, His Tag (Cat. No. EGR-H5222) with a linear range of 0.8-6 ng/mL (QC tested).

Human_FcRn_Heterodimer_Protein_SPR

Immobilized Erbitux on CM5 Chip via anti-human Fc IgG, can bind Human EGF R Protein(Cat.No. EGR-H5222) with an affinity constant of 0.492nM as determined in SPR assay (Biacore T200) ( Routinely tested).

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