This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 70.5kDa. The protein migrates as 110-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human EGF R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Erbitux on CM5 Chip via anti-human Fc IgG, can bind Human EGF R Protein(Cat.No. EGR-H5222) with an affinity constant of 0.492nM as determined in SPR assay (Biacore T200) ( Routinely tested).
Authors: Fazekas-Singer J, et al.
Journal: Oncotarget 2017
Authors: Nath N, et al.
Journal: J Immunol Methods 2017
Authors: Yu X, et al.
Journal: J Immunol Methods 2016
Application: Cell-based assay
Authors: Kant S, et al.
Journal: Mol Microbiol 2015
Application: Assay for Enzyme Activity
Authors: Singer J, et al.
Journal: Mol Cancer Ther 2014
Authors: Olsen DA, et al.
Journal: Clin Chem Lab Med 2013
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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