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Your Position: Home > Isotype Controls > IgG3 Fc > Recombinant Mouse IgG3 Fc Protein

Mouse IgG3 Fc Protein, Tag Free

  • Synonym
    Ig gamma-3 chain C region,IgG, IgG3
  • Source
    Mouse IgG3 Fc, Tag Free (IG3-M5201) is expressed from human 293 cells (HEK293). It contains AA Glu 97 - Lys 329 (Accession # P03987-2).
    Predicted N-terminus: Glu 97
  • Molecular Characterization
    IgG3 Fc(Glu 97 - Lys 329) P03987-2

    This protein carries no "tag".

    The protein has a calculated MW of 26.2 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Mouse IgG3 Fc, Tag Free (Cat. No. IG3-M5201) SDS-PAGE gel

Mouse IgG3 Fc, Tag Free on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Mouse IgG3 Fc, Tag FreeMouse IgG3 Fc, Tag Free (Cat. No. IG3-M5201) ELISA bioactivity

Immobilized Mouse IgG3 Fc, Tag Free (Cat. No. IG3-M5201) at 2 μg/mL (100 μL/well) can bind Human CD64, His Tag (Cat. No. FCA-H52H2) with a linear range of 2-20 ng/mL (QC tested).

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