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Your Position: Home > Receptor Tyrosine Kinases > EGF R > EGR-H82E3

Biotinylated Human EGF R Protein, His,Avitag™ (MALS verified)

INTRODUCTION
QC & Assay Protocol
Citation & Background
  • Synonym
    EGFR,ERBB,ERBB1,HER1,PIG61,mENA
  • Source
    Biotinylated Human EGF R, His,Avitag (EGR-H82E3) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645 (Accession # P00533-1).
    Predicted N-terminus: Leu 25
    Request for sequence
  • Molecular Characterization
    Online(Leu 25 - Ser 645) P00533-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

    The protein has a calculated MW of 72.3 kDa. The protein migrates as 90-125 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 6 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human EGF R, His,Avitag (Cat. No. EGR-H82E3) SDS-PAGE gel

Biotinylated Human EGF R, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Biotinylated Human EGF R, His,Avitag (Cat. No. ) MALS images

The purity of Biotinylated Human EGF R, His,Avitag (Cat. No. EGR-H82E3) was more than 90% and the molecular weight of this protein is around 100-115 kDa verified by SEC-MALS.

Report
Bioactivity-ELISA
Biotinylated Human EGF R, His,AvitagBiotinylated Human EGF R, His,Avitag (Cat. No. EGR-H82E3) ELISA bioactivity

Immobilized Cetuximab at 2 μg/mL (100 μL/well) can bind Biotinylated Human EGF R, His,Avitag (Cat. No. EGR-H82E3) with a linear range of 2-6 ng/mL (QC tested).

Protocol
Biotinylated Human EGF R, His,AvitagBiotinylated Human EGF R, His,Avitag (Cat. No. EGR-H82E3) ELISA bioactivity

Immobilized Human EGF, Tag Free (Cat. No. EGF-H4114) at 10 μg/mL (100 μL/well) can bind Biotinylated Human EGF R, His,Avitag (Cat. No. EGR-H82E3) with a linear range of 0.156-5 μg/mL (Routinely tested).

Protocol
Evaluation of CAR expression
FACS Analysis of biotinylated EGFR binding cell surface anti-EGFR scFv
Evaluation of CAR expression

Cells were transfected with anti-EGFR scFv and cultured for 3 days. Three days post-transfection, 1e6 cells were stained for anti-EGFR scFv with Biotinylated Human EGFR, His,Avitag (Cat. No. EGR-H82E3) followed by PE-conjugated streptavidin. Flow Cytometry assay shows that Biotinylated Human EGFR, His,Avitag (Cat. No. EGR-H82E3) can bind to cells expressing anti-EGFR scFv. The concentration of EGFR used is 0.8 µg/mL. (Data are kindly provided by Guangzhou Bio-gene Technology Co. Ltd.)

Protocol

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Price(USD) : $1380.00

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  • Number of Drugs in Clinical Trials：127 Details
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