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Your Position: Home > Immune Checkpoint Proteins > B7-H3 > Biotinylated Human B7-H3 / CD276 Protein, His Tag, Avi Tag

Biotinylated Human B7-H3 / CD276 Protein, His Tag & Avi Tag (Avitag™)

  • Synonym
    B7-H3,CD276,B7 homolog 3
  • Source
    Biotinylated Human B7-H3, His Tag (B73-H82E6) is expressed from human 293 cells (HEK293). It contains AA Leu 29 - Pro 245 (Accession # Q5ZPR3-2).
    Predicted N-terminus: Leu 29
  • Molecular Characterization
    B7-H3(Leu 29 - Pro 245) Q5ZPR3-2

    This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.

    The protein has a calculated MW of 26.5 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human B7-H3, His Tag (Cat. No. B73-H82E6) SDS-PAGE gel

Biotinylated Human B7-H3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

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Price(USD) : $350.00

Price(USD) : $1380.00

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  • PEGS Boston 2018
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B7-H3 Products

  • Name:CD276 antigen
  • Classification:Homo sapiens
  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:6 Details
  • Lastest Research Phase:Phase ?
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