0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > Immune Checkpoint Proteins > CD27 Ligand > Biotinylated Human CD27 Ligand / CD70 / TNFSF7 Protein, Fc Tag

Biotinylated Human CD27 Ligand / CD70 Protein, Fc Tag, Avi Tag (Avitag™)

  • Synonym
    CD70,CD27LG,TNFSF7,TNFSF7G,CD27L
  • Source
    Biotinylated Human CD27 Ligand, Fc Tag (TN7-H82F4) is expressed from human 293 cells (HEK293). It contains AA Gln 39 - Pro 193 (Accession # P32970-1).
    Predicted N-terminus: Gly
  • Molecular Characterization
    CD27 Ligand(Gln 39 - Pro 193) P32970-1

    This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a human IgG1 Fc tag.

    The protein has a calculated MW of 45 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >85% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human CD27 Ligand, Fc Tag (Cat. No. TN7-H82F4) SDS-PAGE gel

Biotinylated Human CD27 Ligand, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 85%.

Bioactivity
Biotinylated Human CD27 Ligand, Fc TagBiotinylated Human CD27 Ligand, Fc Tag (Cat. No. TN7-H82F4) ELISA bioactivity

Immobilized Human CD27, Fc Tag (Cat. No. CD7-H5254) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD27 Ligand, Fc Tag (Cat. No. TN7-H82F4) with a linear range of 1-31 ng/mL (QC tested).

Comments (0)

Order Details

Price(USD) : $350.00

Price(USD) : $1380.00

Promotion & Exhibitions

  • PEGS Boston 2018
  • WPC Boston 2018

CD27 Ligand Products

  • Name:CD70 antigen
  • Classification:Homo sapiens
  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:8 Details
  • Lastest Research Phase:Phase ?
  • MORE

Datasheet & Documentation

Contact Us

+1  800-810-0816 (US)
+86 400-682-2521 (AP)

Questions & Comments

This web search service is supported by Google Inc.

totop