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Your Position: Home > Immune Checkpoint Proteins > CD27 Ligand > Biotinylated Human CD27 Ligand / CD70 Protein, Avitag,Fc Tag

Biotinylated Human CD27 Ligand / CD70 Protein, Avitag™,Fc Tag

  • Synonym
    CD70,CD27LG,TNFSF7,TNFSF7G,CD27L
  • Source
    Biotinylated Human CD27 Ligand, Avitag,Fc Tag (TN7-H82F4) is expressed from human 293 cells (HEK293). It contains AA Gln 39 - Pro 193 (Accession # P32970-1).
    Predicted N-terminus: Gly
  • Molecular Characterization
    Online(Gln 39 - Pro 193) P32970-1

    This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a human IgG1 Fc tag.

    The protein has a calculated MW of 45 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >85% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human CD27 Ligand, Avitag,Fc Tag (Cat. No. TN7-H82F4) SDS-PAGE gel

Biotinylated Human CD27 Ligand, Avitag,Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 85%.

Bioactivity-ELISA
Biotinylated Human CD27 Ligand, Avitag,Fc TagBiotinylated Human CD27 Ligand, Avitag,Fc Tag (Cat. No. TN7-H82F4) ELISA bioactivity

Immobilized Human CD27, Fc Tag (Cat. No. CD7-H5254) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD27 Ligand, Avitag,Fc Tag (Cat. No. TN7-H82F4) with a linear range of 1-31 ng/mL (QC tested).

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