This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 32.0 kDa. The protein migrates as 47-58 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human CD19 (20-291), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Monoclonal Anti-Human CD19 Antibody, Mouse IgG2a at 2 μg/mL (100 μL/well) can bind Human CD19 (20-291), His Tag (Cat. No. CD9-H52H2) with a linear range of 10-78 ng/mL (QC tested).
FMC63 MAb (Mouse lgG2a) captured on CM5 chip via anti-mouse antibodies surface can bind Human CD19 (20-291), His Tag (Cat. No. CD9-H52H2) with an affinity constant of 2.9 nM as determined in a SPR assay (Biacore 8K) (QC tested).
Loaded Monoclonal Anti-Human CD19 Antibody, Mouse IgG2a on AMC Biosensor, can bind Human CD19 (20-291), His Tag (Cat. No. CD9-H52H2) with an affinity constant of 3.19 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Monoclonal Anti-Human CD19 Antibody, Human IgG1 on AHC Biosensor, can bind Human CD19 (20-291), His Tag (Cat. No. CD9-H52H2) with an affinity constant of 4.28 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
2e5 of anti-CD19 CAR-293 cells were stained with 100 μL of 10 μg/mL of Human CD19 (20-291), His Tag (Cat. No. CD9-H52H2) and negative control protein respectively, washed and then followed by FITC anti-His tag antibody and analyzed with FACS (Routinely tested).
Authors: Zhang A, Sun Y, Wang S, et al.
Journal: Cytotherapy 2020
Application: Flow cytometry
Authors: Lobb Roy, et al.
Journal: US20180142035A1 2018
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