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Your Position: Home > Cytokines > IL-17A & IL-17F > Human IL-17A & IL-17F Heterodimer Protein

Human IL-17A & IL-17F Heterodimer Protein

  • Synonym
    IL-17A,Interleukin-17A,CTLA-8,IL-17,IL-17F,IL24,ML-1,Interleukin-17F
  • Source
    Human IL-17A & IL-17F Heterodimer Protein (ILF-H52WF) is expressed from human 293 cells (HEK293). It contains AA Gly 24 - Ala 155 (IL-17A) & Arg 31 - Gln 163 (IL-17F) (Accession # Q16552-1 (IL-17A) & Q96PD4-1 (IL-17F)). Two sequential affinity purification steps were used to ensure exact 1:1 molar ratio of IL17A &IL17F heterodimer.
    Predicted N-terminus: His (IL-17A) & Ser (IL-17F)
  • Molecular Characterization
    IL-17A & IL-17F(Gly 24 - Ala 155 (IL-17A) & Arg 31 - Gln 163 (IL-17F)) Q16552-1 (IL-17A) & Q96PD4-1 (IL-17F)

    Human IL-17A & IL-17F Heterodimer Protein, produced by co-expression of IL-17A and IL-17F, has a calculated MW of 17.0 kDa (IL-17A) and 18.4 kDa (IL-17F). Subunit IL-17A is fused with a polyhistidine tag at the N-terminus and subunit IL-17F is fused with a Twin Strep tag at the N-terminus. The predicted N-terminus is His (IL-17A) & Ser (IL-17F). The reducing (R) protein migrates as 19 kDa (IL-17A) and 21 kDa (IL-17F) respectively due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by reduced SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human IL-17A & IL-17F Heterodimer Protein (Cat. No. ILF-H52WF) SDS-PAGE gel

Human IL-17A & IL-17F Heterodimer Protein on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Human IL-17A & IL-17F Heterodimer ProteinHuman IL-17A & IL-17F Heterodimer Protein (Cat. No. ILF-H52WF) ELISA bioactivity

Immobilized Human IL-17A & IL-17F, His Tag, Strep Tag (Cat. No. ILF-H52WF) at 2 μg/mL (100 μL/well) can bind Human IL-17 RA, Fc Tag (Cat. No. ILA-H5257) with a linear range of 39-625 ng/mL (QC tested).

Human IL-17A & IL-17F Heterodimer ProteinHuman IL-17A & IL-17F Heterodimer Protein (Cat. No. ILF-H52WF) ELISA bioactivity

Immobilized Anti-IL17A Mab Human IgG1 at 2 μg/mL (100 μL/well) can bind Human IL-17A & IL-17F, His Tag, Strep Tag (Cat. No. ILF-H52WF) with a linear range of 0.2-3 ng/mL (Routinely tested).

  • Background
    Interleukin-17A (IL17A) is also known as cytotoxic T-lymphocyte-associated antigen 8 (CTLA8),which is a proinflammatory cytokine produced by activated T cells. IL17A can regulate the activities of NF-kappaB and mitogen-activated protein kinases. Also,IL17A can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO).Furthermore,IL17A has been found both in glycosylated and nonglycosylated forms. High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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