Human IL-17A&IL-17F Heterodimer Protein, His Tag&Strep II Tag, produced by co-expression of IL-17A and IL-17F, has a calculated MW of 17.0 kDa (IL-17A) and 18.4 kDa (IL-17F). Subunit IL-17A is fused with a polyhistidine tag at the N-terminus and subunit IL-17F is fused with a Twin Strep tag at the N-terminus. The reducing (R) protein migrates as 19 kDa (IL-17A) and 21 kDa (IL-17F) respectively due to glycosylation.
>95% as determined by reduced SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human IL-17A&IL-17F Heterodimer Protein, His Tag&Strep II Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human IL-17A&IL-17F Heterodimer Protein, His Tag&Strep II Tag (Cat. No. ILF-H52WF) at 2 μg/mL (100 μL/well) can bind Human IL-17 RA, Fc Tag (Cat. No. ILA-H5257) with a linear range of 0.039-0.625 μg/mL (QC tested).
Loaded Human IL-17 RA, Fc Tag (Cat. No. ILA-H5257) on Protein A Biosensor, can bind Human IL-17A&IL-17F Heterodimer Protein, His Tag&Strep II Tag (Cat. No. ILF-H52WF) with an affinity constant of 34.4 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Human IL-17A&IL-17F Heterodimer Protein, His Tag&Strep II Tag (Cat. No. ILF-H52WF) stimulates secretion of IL-6 by NIH-3T3 cells. The ED50 for this effect is 3.14-9.01 ng/mL (Routinely tested).
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