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Your Position: Home > Recombinant Proteins > Human Integrin alpha E beta 7 (ITGAE&ITGB7) Heterodimer Protein

Human Integrin alpha E beta 7 (ITGAE&ITGB7) Heterodimer Protein

  • Synonym
    Integrin alpha E beta 7,ITGAE&ITGB7,HML-1,CD103
  • Source
    Human ITGAE & ITGB7 Heterodimer Protein (IT7-H52W7) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Ser 1124 (ITGAE) & Glu 20 - His 723 (ITGB7) (Accession # P38570-1 (ITGAE) & P26010-1 (ITGB7)).
    Predicted N-terminus: Phe 19 (ITGAE) & Glu 20 (ITGB7)
  • Molecular Characterization
    ITGAE&ITGB7(Phe 19 - Ser 1124 (ITGAE) & Glu 20 - His 723 (ITGB7)) P38570-1 (ITGAE) & P26010-1 (ITGB7)

    Human ITGAE & ITGB7 Heterodimer Protein, produced by co-expression of ITGAE and ITGB7, has a calculated MW of 128.6 kDa (ITGAE) and 81.8 kDa (ITGB7). Subunit ITGAE is fused with a polyhistidine tag at the C-terminus and subunit ITGB7 contains no tag. The predicted N-terminus is Phe 19 (ITGAE) & Glu 20 (ITGB7). The reducing (R) protein migrates as 135-150 kDa (ITGAE) and 105-115 kDa (ITGB7) respectively due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by reduced SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human ITGAE & ITGB7 Heterodimer Protein (Cat. No. IT7-H52W7) SDS-PAGE gel

Human ITGAE & ITGB7 Heterodimer Protein on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity
Human ITGAE & ITGB7 Heterodimer ProteinHuman ITGAE & ITGB7 Heterodimer Protein (Cat. No. IT7-H52W7) ELISA bioactivity

Immobilized Human ITGAE&ITGB7, His Tag (Cat. No. IT7-H52W7) at 5 μg/mL (100 μL/well) can bind Human E-Cadherin, Fc Tag (Cat. No. ECD-H5250) with a linear range of 0.08-2.5 μg/mL (QC tested).

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Price(USD) : $350.00

Inventory : In stock

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