0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > All Other Proteins > Integrin alpha V beta 6 > Biotinylated Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein

Biotinylated Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein

  • Synonym
    Integrin alpha V beta 6,ITGAV&ITGB6
  • Source
    Biotinylated Human ITGAV & ITGB6 Heterodimer Protein (IT6-H82E4) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Val 992 (ITGAV) & Gly 22 - Asn 707 (ITGB6) (Accession # NP_002201.1 (ITGAV)& AAI21179.1 (ITGB6)).
    Predicted N-terminus: Phe 31 (ITGAV) & Gly 22 (ITGB6)
  • Molecular Characterization
    ITGAV&ITGB6(Phe 31 - Val 992 (ITGAV) & Gly 22 - Asn 707 (ITGB6)) NP_002201.1 (ITGAV)& AAI21179.1 (ITGB6)

    Biotinylated Human ITGAV & ITGB6 Heterodimer Protein, produced by co-expression of ITGAV and ITGB6, has a calculated MW of 113 kDa (ITGAV) and 79.6 kDa (ITGB6). Subunit ITGAV is  fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB6 contains no tag but a basic tail at the C-terminus. The predicted N-terminus is Phe 31 (ITGAV) & Gly 22 (ITGB6). The reducing (R) protein migrates as 100-140 kDa (ITGAV) and 145-160 kDa (ITGB6) respectively due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by reduced SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, 2 mM CaCl2, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Biotinylated Human ITGAV & ITGB6 Heterodimer Protein (Cat. No. IT6-H82E4) SDS-PAGE gel

Biotinylated Human ITGAV & ITGB6 Heterodimer Protein on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity
Biotinylated Human ITGAV & ITGB6 Heterodimer ProteinBiotinylated Human ITGAV & ITGB6 Heterodimer Protein (Cat. No. IT6-H82E4) ELISA bioactivity

Immobilized Human TGFB1, His Tag (Cat. No. TG1-H524x) at 5 μg/mL (100 μL/well) can bind Biotinylated Human ITGAV&ITGB6, His Tag, Avi Tag (Cat. No. IT6-H82E4) with a linear range of 2-10 ng/mL (QC tested).

Biotinylated Human ITGAV & ITGB6 Heterodimer ProteinBiotinylated Human ITGAV & ITGB6 Heterodimer Protein (Cat. No. IT6-H82E4) ELISA bioactivity

Immobilized Fibronectin at 10 μg/mL (100 μL/well) can bind Biotinylated Human ITGAV&ITGB6, His Tag, Avi Tag (Cat. No. IT6-H82E4) with a linear range of 0.08-1.25 μg/mL (Routinely tested).

  • Background
    Integrin alpha V beta 6 is a heterodimer of beta-6 associating with alpha-V. Integrin alpha-V beta-6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of integrin alpha-V beta-6 via clathrin-mediated endocytosis promotes carcinoma cell invasion. Also, Integrin alpha-V beta-6 acts as a receptor for coxsackievirus A9 and coxsackievirus B1 as well as herpes simplex virus-1/HHV-1. Furthermore, it binds the TGF-beta latency‑associated peptide (LAP) and activates TGF-beta 1 or TGF-beta 3 from large latent complexes. This activation requires interaction with LTBP-1 and fibronectin, and is enhanced by PAR-1.
  • References
  • Limited Use License

    The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

    Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

Comments (0)

Order Details

Price(USD) : $350.00

Price(USD) : $1380.00

Promotion & Exhibitions

  • PEGS Boston 2018
  • WPC Boston 2018

Datasheet & Documentation

Contact Us

+1  800-810-0816 (US)
+86 400-682-2521 (AP)

Questions & Comments

This web search service is supported by Google Inc.

totop