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Your Position: Home > Recombinant Proteins > CD28 > Human / Cynomolgus / Rhesus macaque CD28, mouse IgG2a Fc tag

Human / Cynomolgus / Rhesus macaque CD28 Protein, Mouse IgG2a Fc Tag, low endotoxin

  • Synonym
    CD28,Tp44
  • Source
    Human / Cynomolgus / Rhesus macaque CD28, mouse IgG2a Fc tag (CD8-H52A5) is expressed from human 293 cells (HEK293). It contains AA Asn 19 - Pro 152 (Accession # P10747-1).
    Predicted N-terminus: Asn 19
  • Molecular Characterization
    CD28(Asn 19 - Pro 152) P10747-1

    This protein carries a mouse IgG2a Fc tag at the C-terminus.

    The protein has a calculated MW of 42.0 kDa. The protein migrates as 50-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human / Cynomolgus / Rhesus macaque CD28, mouse IgG2a Fc tag (Cat. No. CD8-H52A5) SDS-PAGE gel

Human / Cynomolgus / Rhesus macaque CD28, mouse IgG2a Fc tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Human / Cynomolgus / Rhesus macaque CD28, mouse IgG2a Fc tagHuman / Cynomolgus / Rhesus macaque CD28, mouse IgG2a Fc tag (Cat. No. CD8-H52A5) ELISA bioactivity

Immobilized Human / Cynomolgus / Rhesus macaque CD28, mouse IgG2a Fc tag (Cat. No. CD8-H52A5) at 5 μg/mL (100 μL/well) can bind Human B7-2, Fc Tag (Cat. No. CD6-H5257) with a linear range of 0.313-5 μg/mL (QC tested).

Human / Cynomolgus / Rhesus macaque CD28, mouse IgG2a Fc tagHuman / Cynomolgus / Rhesus macaque CD28, mouse IgG2a Fc tag (Cat. No. CD8-H52A5) ELISA bioactivity

Immobilized Human / Cynomolgus / Rhesus macaque CD28, mouse IgG2a Fc tag (Cat. No. CD8-H52A5) at 5 μg/mL (100 μL/well) can bind Human B7-1, Fc Tag (Cat. No. B71-H5259) with a linear range of 0.625-10 μg/mL (Routinely tested).

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