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Your Position: Home > Recombinant Proteins > CD3E & CD3D > Biotinylated Human CD3E & CD3D Heterodimer Protein

Biotinylated Human CD3 epsilon & CD3 delta Heterodimer Protein (Avitag™)

  • Synonym
    CD3E & CD3D,CD3 delta & CD3 epsilon,CD3 delta / epsilon
  • Source
    MABSol® Biotinylated Human CD3 epsilon & CD3 delta (CDD-H82W1) is expressed from human HEK293 cells. It contains AA Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # P07766-1 (CD3E) & P04234-1 (CD3D)). It is the biotinylated form of Human CD3 epsilon & CD3 delta Protein.
    Predicted N-terminus: Asp 23 (CD3E) & Phe 22 (CD3D)
  • Molecular Characterization
    CD3E & CD3D(Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D)) P07766-1 (CD3E) & P04234-1 (CD3D)

    MABSol® Biotinylated Human CD3 epsilon & CD3 delta Heterodimer Protein is produced by co-expression of CD3E and CD3D, has a calculated MW of 18.7 kDa (CD3E), 13.5 kDa (CD3D) . Subunit CD3E is fused with a polyhistidine tag and a Avi tag at the C-terminus and subunit CD3D contains no tag. The predicted N-terminus is Asp 23 (CD3E) & Phe 22 (CD3D). The reducing (R) heterodimer protein migrates as 22 kDa (CD3E) and 26 kDa (CD3D) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Biotinylated Human CD3E & CD3D (Cat. No. CDD-H82W1) SDS-PAGE gel

Biotinylated Human CD3E & CD3D on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity
Biotinylated Human CD3E & CD3DBiotinylated Human CD3E & CD3D (Cat. No. CDD-H82W1) ELISA bioactivity

Immobilized OKT3 at 10 μg/mL (100 μL/well) can bind Biotinylated Human CD3E & CD3D (Cat. No. CDD-H82W1) with a linear range of 0.156-5 μg/mL (QC tested).

  • Background
    T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
  • References
  • Limited Use License

    The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

    Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $1380.00

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