This is the new website for acrobiosystems.com. We would love to hear what you think of it - please send feedback

Welcome to ACROBiosystems

0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > Recombinant Proteins > Human B7-1 / CD80 Protein, mouse IgG2a Fc Tag, low endotoxin

Human B7-1 / CD80 Protein, Mouse IgG2a Fc Tag, low endotoxin

  • Synonym
    CD80,B7,B7-1,B7.1,BB1,CD28LG,CD28LG1,LAB7
  • Source
    Human B7-1, mouse IgG2a Fc Tag (B71-H52A4) is expressed from human 293 cells (HEK293). It contains AA Val 35 - Asn 242 (Accession # P33681-1).
    Predicted N-terminus: Val 35
  • Molecular Characterization
    B7-1(Val 35 - Asn 242) P33681-1

    This protein carries a mouse IgG2a Fc tag at the C-terminus.

    The protein has a calculated MW of 50.9 kDa. The protein migrates as 65-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human B7-1, mouse IgG2a Fc Tag (Cat. No. B71-H52A4) SDS-PAGE gel

Human B7-1, mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Human B7-1, mouse IgG2a Fc TagHuman B7-1, mouse IgG2a Fc Tag (Cat. No. B71-H52A4) ELISA bioactivity

Immobilized Human CTLA-4, Fc Tag (Cat. No. CT4-H5255) at 1 μg/mL (100 μL/well) can bind Human B7-1, mouse IgG2a Fc Tag (Cat. No. B71-H52A4) with a linear range of 3-13 ng/mL (QC tested).

  • Background
    B7-1 and B7-2, together with their receptors CD28 and CTLA­4, constitute one of the dominant co-stimulatory pathways that regulate T­ and B­cell responses. Although both CTLA­4 and CD28 can bind to the same ligands, CTLA­4 binds to B7­1 and B7­2 with a 20 ­ 100 fold higher affinity than CD28 and is involved in the down­regulation of the immune response. B-lymphocyte activation antigen B7-1 (referred to as B7) also known as cluster of Differentiation 80 (CD80), is a member of cell surface immunoglobulin superfamily and is expressed on activated B cells, activated T cells, macrophages and dendritic cells. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. CD80 plays a role in induction of innate immune responses by activating NF-κB-signaling pathway in macrophages. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

Comments (0)

Order Details

Price(USD) : $260.00

Inventory : In stock

Price(USD) : $1760.00

Inventory : In stock

Datasheet & Documentation

Contact Us

+1  800-810-0816 (US)
+86 400-682-2521 (AP)

Related Products

Questions & Comments

This web search service is supported by Google Inc.