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Your Position: Home > Recombinant Proteins > Human Integrin alpha 8 beta 1 (ITGA8&ITGB1) Heterodimer Protein

Human Integrin alpha 8 beta 1 (ITGA8&ITGB1) Heterodimer Protein

  • Synonym
    Integrin alpha 8 beta 1,ITGA8&ITGB1,ITGA8&B1
  • Source
    Recombinant Human ITGA8 & ITGB1 Heterodimer Protein (IT1-H52W9) is expressed from human 293 cells (HEK293). It contains AA Phe 39 - Leu 1012 (ITGA8) & Gln 21 - Asp 728 (ITGB1) (Accession # P53708-1 (ITGA8) & P05556-1 (ITGB1)).
    Predicted N-terminus: Phe 39 (ITGA8) & Gln 21 (ITGB1)
  • Molecular Characterization
    ITGA8&ITGB1(Phe 39 - Leu 1012 (ITGA8) & Gln 21 - Asp 728 (ITGB1)) P53708-1 (ITGA8) & P05556-1 (ITGB1)

    Human ITGA8 & ITGB1 Heterodimer Protein, produced by co-expression of ITGA8 and ITGB1, has a calculated MW of 114.6 kDa (ITGA5) & 83.7 kDa (ITGB1). Subunit ITGA8 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The predicted N-terminus is Phe 39 (ITGA8) & Gln 21 (ITGB1). The reducing (R) protein migrates as 115-155 kDa due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human ITGA8&ITGB1 Heterodimer Protein (Cat. No. IT1-H52W9) SDS-PAGE gel

Human ITGA8&ITGB1 Heterodimer Protein on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

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