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Your Position: Home > Recombinant Proteins > HVEM > Human HVEM / TNFRSF14 Protein, mouse IgG2a Fc Tag, low endotoxin

Human HVEM / TNFRSF14 Protein, Mouse IgG2a Fc Tag, low endotoxin

  • Synonym
    TNFRSF14,ATAR,HVEA,HVEM,LIGHTR,TR2,CD270
  • Source
    Human HVEM, mouse IgG2a Fc Tag (HVM-H5255) is expressed from human 293 cells (HEK293). It contains AA Leu 39 -Val 202 (Accession # Q92956-1).
    Predicted N-terminus: Leu 39
  • Molecular Characterization
    HVEM(Leu 39 -Val 202) Q92956-1

    This protein carries a mouse IgG2a Fc tag at the C-terminus.

    The protein has a calculated MW of 44.4 kDa. The protein migrates as 55-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Human HVEM, mouse IgG2a Fc Tag (Cat. No. HVM-H5255) SDS-PAGE gel

Human HVEM, mouse IgG2a Fc Tag on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Human HVEM, mouse IgG2a Fc TagHuman HVEM, mouse IgG2a Fc Tag (Cat. No. HVM-H5255) ELISA bioactivity

Immobilized Human HVEM, mouse IgG2a Fc Tag (Cat. No. HVM-H5255) at 5 μg/mL (100 μL/well) can bind Human BTLA, Fc Tag (Cat. No. BTA-H5255) with a linear range of 0.156-1.25 μg/mL (QC tested).

  • Background
    Herpesvirus entry mediator (HVEM) is also known as TNFRSF14, TR2 (TNF receptorlike molecule) and ATAR (another TRAF associated receptor), is a type I membrane protein belonging to the TNF/NGF receptor superfamily.  HVEM expression has been detected in peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells. The extracellular domain of HVEM has been shown to interact directly with the herpes simplex virus envelope glycoprotein D (gD). Two TNF superfamily ligands, including the secreted TNFβ (lymphotoxin α) and the membrane protein LIGHT (lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes), have been shown to be the cellular ligands for HVEM.  Besides HVEM, LIGHT can also interact with LTβR, the receptor for lymphotoxin αβ heterotrimer. The role of the HVEM LIGHT /LTβ receptor ligand pair in immune function and herpesvirus pathobiology remains to be elucidated.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $300.00

Price(USD) : $1980.00

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