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This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 25.7 kDa. The protein migrates as 36-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Cynomolgus CD16, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Cynomolgus CD16, His,Avitag (Cat. No. FC6-C82E0) is more than 90% and the molecular weight of this protein is around 45-55 kDa verified by SEC-MALS.
Immobilized Rituximab at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus CD16, His,Avitag (Cat. No. FC6-C82E0) with a linear range of 0.039-1.25 μg/mL (QC tested).
Captured Biotinylated Cynomolgus CD16, His,Avitag (Cat. No. FC6-C82E0) on Biotin CAP - Series S sensor Chip can bind MabThera® (Rituximab) with an affinity constant of 0.63 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
Loaded Biotinylated Cynomolgus CD16, His,Avitag (Cat. No. FC6-C82E0) on SA Biosensor, can bind Rituximab with an affinity constant of 0.401 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
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